Papers 【 display / non-display 】

Papers 【 display / non-display 】

• Complex formation of immunoglobulin superfamily molecules Side-IV and Beat-IIb regulates synaptic specificity

  43 ( 2 )   2024.2  [Reviewed]

  DOI

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  Language：English   Publishing type：Research paper (scientific journal)   Single Work  

• Cell adhesion and actin dynamics factors promote axonal extension and synapse formation in transplanted Drosophila photoreceptor cells

  Development, Growth & Differentiation   66   205 - 218   2024.1  [Reviewed]

  DOI

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  Language：English   Publishing type：Research paper (scientific journal)   Single Work  

• Drosophila model to clarify the pathological significance of OPA1 in autosomal dominant optic atrophy

  Yohei Nitta , Jiro Osaka , Ryuto Maki , Satoko Hakeda-Suzuki , Emiko Suzuki , Satoshi Ueki , … Show more authors

  eLife   2023.6  [Reviewed]

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  Language：English   Publishing type：Research paper (scientific journal)   Joint Work  

• Direct evaluation of neuroaxonal degeneration with the causative genes of neurodegenerative diseases in <i>Drosophila</i> using the automated axon quantification system, MeDUsA

  Nitta, Y; Kawai, H; Maki, R; Osaka, J; Hakeda-Suzuki, S; Nagai, Y; Doubková, K; Uehara, T; Watanabe … Show more authors

  HUMAN MOLECULAR GENETICS   32 ( 9 )   1524 - 1538   2023.4  [Reviewed]

  DOI Web of Science PubMed CiNii Research

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  Language：The in addition, foreign language   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)   Joint Work  

  <jats:title>Abstract</jats:title>
  <jats:p>Drosophila is an excellent model organism for studying human neurodegenerative diseases (NDs). However, there is still almost no experimental system that could directly observe the degeneration of neurons and automatically quantify axonal degeneration. In this study, we created MeDUsA (a ‘method for the quantification of degeneration using fly axons’), a standalone executable computer program based on Python that combines a pre-trained deep-learning masking tool with an axon terminal counting tool. This software automatically quantifies the number of retinal R7 axons in Drosophila from a confocal z-stack image series. Using this software, we were able to directly demonstrate that axons were degenerated by the representative causative genes of NDs for the first time in Drosophila. The fly retinal axon is an excellent experimental system that is capable of mimicking the pathology of axonal degeneration in human NDs. MeDUsA rapidly and accurately quantifies axons in Drosophila photoreceptor neurons. It enables large-scale research into axonal degeneration, including screening to identify genes or drugs that mediate axonal toxicity caused by ND proteins and diagnose the pathological significance of novel variants of human genes in axons.</jats:p>

• Glial insulin regulates cooperative or antagonistic Golden goal/Flamingo interactions during photoreceptor axon guidance

  Takechi, H; Hakeda-Suzuki, S; Nitta, Y; Ishiwata, Y; Iwanaga, R; Sato, M; Sugie, A; Suzuki, T

  ELIFE   10   2021.3

  DOI Web of Science PubMed CiNii Research

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  Language：The in addition, foreign language   Publishing type：Research paper (scientific journal)   Publisher：eLife Sciences Publications, Ltd   Joint Work  

  <jats:p>Transmembrane protein Golden goal (Gogo) interacts with atypical cadherin Flamingo (Fmi) to direct R8 photoreceptor axons in the<jats:italic>Drosophila</jats:italic>visual system. However, the precise mechanisms underlying Gogo regulation during columnar- and layer-specific R8 axon targeting are unknown. Our studies demonstrated that the insulin secreted from surface and cortex glia switches the phosphorylation status of Gogo, thereby regulating its two distinct functions. Non-phosphorylated Gogo mediates the initial recognition of the glial protrusion in the center of the medulla column, whereas phosphorylated Gogo suppresses radial filopodia extension by counteracting Flamingo to maintain a one axon-to-one column ratio. Later, Gogo expression ceases during the midpupal stage, thus allowing R8 filopodia to extend vertically into the M3 layer. These results demonstrate that the long- and short-range signaling between the glia and R8 axon growth cones regulates growth cone dynamics in a stepwise manner, and thus shapes the entire organization of the visual system.</jats:p>

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Grant-in-Aid for Scientific Research 【 display / non-display 】

Grant-in-Aid for Scientific Research 【 display / non-display 】

• シナプス形成による神経軸索投射と回路網の決定機構

  Grant number：24K09467  2024.4 - 2028.3

  Grant-in-Aid for Scientific Research(C)

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  Authorship：Principal investigator  Grant type：Competitive

• 神経回路の形成における神経間認識の分子機構

  Grant number：21K06184  2021.4 - 2022.4

  Grant-in-Aid for Scientific Research(C)

• Molecular codes which determine the depth of final axonal stabilizing layer in the Drosophila

  Grant number：18K06250  2018.3 - 2021.3

  Grant-in-Aid for Scientific Research(C)

• Temporal control of molecular function in axon wiring of the visual system

  Grant number：26440119  2014.4 - 2017.3

  Grant-in-Aid for Scientific Research(C)

  Investigator(s)：Satoko Hakeda-Suzuki

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  Authorship：Principal investigator  Grant type：Competitive