Details of a Researcher - HAKEDA-SUZUKI Satoko

Papers - HAKEDA-SUZUKI Satoko

about 28

Drosophila model to clarify the pathological significance of OPA1 in autosomal dominant optic atrophy

Yohei Nitta, Jiro Osaka, Ryuto Maki, Satoko Hakeda-Suzuki, Emiko Suzuki, Satoshi Ueki, Takashi Suzu … Show more authors

eLife 2024.8 [Reviewed]

Language：English Publishing type：Research paper (scientific journal) Single Work

Cell adhesion and actin dynamics factors promote axonal extension and synapse formation in transplanted Drosophila photoreceptor cells

Riku Iwanaga; Nagisa Yahagi; Satoko Hakeda‐Suzuki; Takashi Suzuki

Development, Growth & Differentiation 2024.2 [Reviewed]

DOI

Language：English Publishing type：Research paper (scientific journal) Single Work

Complex formation of immunoglobulin superfamily molecules Side-IV and Beat-IIb regulates synaptic specificity

2024.2 [Reviewed]

DOI

Language：English Publishing type：Research paper (scientific journal) Single Work

Direct evaluation of neuroaxonal degeneration with the causative genes of neurodegenerative diseases in Drosophila using the automated axon quantification system, MeDUsA

Nitta, Y; Kawai, H; Maki, R; Osaka, J; Hakeda-Suzuki, S; Nagai, Y; Doubková, K; Uehara, T; Watanabe … Show more authors

HUMAN MOLECULAR GENETICS 32 ( 9 ) 1524 - 1538 2023.4 [Reviewed]

DOI Web of Science PubMed CiNii Research

Language：The in addition, foreign language Publishing type：Research paper (scientific journal) Publisher：Oxford University Press (OUP) Joint Work

<jats:title>Abstract</jats:title>
<jats:p>Drosophila is an excellent model organism for studying human neurodegenerative diseases (NDs). However, there is still almost no experimental system that could directly observe the degeneration of neurons and automatically quantify axonal degeneration. In this study, we created MeDUsA (a ‘method for the quantification of degeneration using fly axons’), a standalone executable computer program based on Python that combines a pre-trained deep-learning masking tool with an axon terminal counting tool. This software automatically quantifies the number of retinal R7 axons in Drosophila from a confocal z-stack image series. Using this software, we were able to directly demonstrate that axons were degenerated by the representative causative genes of NDs for the first time in Drosophila. The fly retinal axon is an excellent experimental system that is capable of mimicking the pathology of axonal degeneration in human NDs. MeDUsA rapidly and accurately quantifies axons in Drosophila photoreceptor neurons. It enables large-scale research into axonal degeneration, including screening to identify genes or drugs that mediate axonal toxicity caused by ND proteins and diagnose the pathological significance of novel variants of human genes in axons.</jats:p>

Glial insulin regulates cooperative or antagonistic Golden goal/Flamingo interactions during photoreceptor axon guidance

Takechi, H; Hakeda-Suzuki, S; Nitta, Y; Ishiwata, Y; Iwanaga, R; Sato, M; Sugie, A; Suzuki, T

ELIFE 10 2021.3

DOI Web of Science PubMed CiNii Research

Language：The in addition, foreign language Publishing type：Research paper (scientific journal) Publisher：eLife Sciences Publications, Ltd Joint Work

<jats:p>Transmembrane protein Golden goal (Gogo) interacts with atypical cadherin Flamingo (Fmi) to direct R8 photoreceptor axons in the<jats:italic>Drosophila</jats:italic>visual system. However, the precise mechanisms underlying Gogo regulation during columnar- and layer-specific R8 axon targeting are unknown. Our studies demonstrated that the insulin secreted from surface and cortex glia switches the phosphorylation status of Gogo, thereby regulating its two distinct functions. Non-phosphorylated Gogo mediates the initial recognition of the glial protrusion in the center of the medulla column, whereas phosphorylated Gogo suppresses radial filopodia extension by counteracting Flamingo to maintain a one axon-to-one column ratio. Later, Gogo expression ceases during the midpupal stage, thus allowing R8 filopodia to extend vertically into the M3 layer. These results demonstrate that the long- and short-range signaling between the glia and R8 axon growth cones regulates growth cone dynamics in a stepwise manner, and thus shapes the entire organization of the visual system.</jats:p>

Activity-dependent endocytosis of Wingless regulates synaptic plasticity in the Drosophila visual system

Kawamura, H; Hakeda-Suzuki, S; Suzuki, T

GENES & GENETIC SYSTEMS 95 ( 5 ) 235 - 247 2020.10 [Reviewed]

<HAKEDA-SUZUKI Satoko>

Papers - HAKEDA-SUZUKI Satoko