Papers - HIRATSUKA Kazuyuki
about 59-
Studies on regulated expression of plant defense genes
Kazuyuki HIratsuka
Journal of General Plant Pathology 86 ( 6 ) 531 - 533 2020.10 [Reviewed] [Invited]
Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Nature Single Work
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Suppression of defense gene expression under nutrient-rich condition in Arabidopsis seedlings
Nakamura T, Osawa Y, Ogura R, Hiratsuka K
Plant biotechnology 41 479 - 483 2024.7 [Reviewed]
Authorship：Corresponding author Language：English Publishing type：Research paper (scientific journal) Single Work
Other Link： https://www.jstage.jst.go.jp/article/plantbiotechnology/41/4/41_24.0726a/_article/-char/ja/
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Front. Bioeng. Biotechnol., 12 2024.2 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Single Work
Other Link： https://www.frontiersin.org/articles/10.3389/fbioe.2024.1359388/full
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Sueda K, Nanya K, Uyeda I, Tasaka Y, Ogura R, Hiratsuka K, Matsumura T
Current Plant Biology 30 100245 2022.6 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Single Work
Other Link： https://doi.org/10.1016/j.cpb.2022.100245
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Studies on regulated expression of plant defense genes
HIRATSUKA K.
Japanese Journal of Phytopathology 86 ( 4 ) 252 - 255 2020.11
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：The Phytopathological Society of Japan Single Work
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Hiroyuki Hagiwara, Rieko Ogura, Takeshi Fukumoto, Toshiaki Ohara, Mikio Tsuda, Kazuyuki Hiratsuka
Journal of General Plant Pathology 86 ( 1 ) 39 - 47 2019.11 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
The fungicide tolprocarb (TPC) is a melanin biosynthesis inhibitor, but it may also have another mode of action. Here in tests of TPC for inducing plant systemic acquired resistance (SAR), TPC induced promoter activity of the tobacco pathogenesis-related gene PR-1a in Arabidopsis thaliana and genes for PBZ1, β-1,3-glucanase, and chitinase 1 in the defense-related salicylic acid (SA) signaling pathway in rice, but not genes for the jasmonate signaling pathway. Probenazole (PBZ), a commercially used plant defense activator, induced genes in both signaling pathways. The antibacterial activity of TPC was equivalent to that of PBZ. Irrigation with 200 μM TPC prevented growth by Pseudomonas syringae pv. maculicola in A. thaliana, and 30 μM TPC inhibited Xanthomonas oryzae pv. oryzae growth in rice. The results of this study suggest that TPC functions not only as a melanin biosynthesis inhibitor but also as an SAR inducer and is applicable as a novel bacterial control agent that induces SAR activity in both A. thaliana and rice.
Other Link： https://link.springer.com/article/10.1007%2Fs10327-019-00891-5
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Development of a protein-luciferase-based high-throughput screening system to monitor degradation of Jasmonate ZIM-domain family proteins
Ishida H., Ogura R., Hiratsuka K.
PHYTOPATHOLOGY 108 ( 10 ) 13 - 13 2018.10 [Reviewed]
Language：Japanese Publishing type：Research paper (scientific journal) Joint Work
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Chloroplast DNA phylogeography of Sanguisorba officinalis : an indicator of the Satoyama lands
SAEKI Ikuyo,IIDA Shinya,KOIKE Fumito,KOBAYASHI Yoshiko,HIRATSUKA Kazuyuki
Journal of the Japanese Society of Revegetation Technology 38 ( 1 ) 115 - 120 2012
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOCIETY OF REVEGETATION TECHNOLOGY Joint Work
Other Link： https://www.jstage.jst.go.jp/article/jjsrt/38/1/38_115/_article/-char/ja/
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Development of a promoter-luciferase-based high-throughput system to monitor jasmonate-mediated defense gene expression.
Kusama M, Urata N, Ogura R, Ogata S, Hiratsuka K
Plant biotechnol. 29 515 - 520 2012 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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Novel intron-containing luciferase genes for quantitative analysis of mRNA levels in transient gene expression assays.
Hayakawa H, Ogura R, Hiratsuka K, Suzuki M, Ugaki M
Plant biotechnol. 29 ( 5 ) 505 - 509 2012 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOC PLANT CELL & MOLECULAR BIOLOGY Joint Work
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In vivo bioluminescence monitoring of defense gene expression in response to treatment with yeast cell wall extract.
Minami T, Tanaka T, Takasaki S, Kawamura K, Hiratsuka K
Plant biotechnology 28 481 - 484 2011.12 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOC PLANT CELL & MOLECULAR BIOL Joint Work
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Bioluminescence spectra of click beetle luciferases in higher plant cells.
Ogura R, Matsuo N
Plant biotechnol. 28 ( 4 ) 423 - 426 2011
Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOC PLANT CELL & MOLECULAR BIOL Joint Work
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Evaluation of the use of the tobacco PR-1a promoter to monitor defense gene expression by the luciferase bioluminescence reporter system.
Ono S, Kusama M, Ogura R
Biosci. Biotechnol. Biochem. 75 ( 9 ) 1796 - 1800 2011
Language：English Publishing type：Research paper (scientific journal) Publisher：TAYLOR & FRANCIS LTD Joint Work
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Non-destructive bioluminescence detection system for monitoring defense gene expression in tobacco BY-2 cells.
Watakabe Y, Ono S, Tanaka T
Plant biotechnol. 28 ( 3 ) 295 - 301 2011
Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOC PLANT CELL & MOLECULAR BIOL Joint Work
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Nagata, T; Niyada, E; Fujimoto, N; Nagasaki, Y; Noto, K; Miyanoiri, Y; Murata, J; Hiratsuka, K; Kat … Show more authors
Nagata, T; Niyada, E; Fujimoto, N; Nagasaki, Y; Noto, K; Miyanoiri, Y; Murata, J; Hiratsuka, K; Katahira, M Hide authors
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS 78 ( 14 ) 3033 - 3047 2010.11 [Reviewed]
DOI Web of Science Scopus PubMed
Language：The in addition, foreign language Publishing type：Research paper (scientific journal) Joint Work
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Overall linkage map of the nonstructural proteins of Aichi virus.
Ishikawa, K., Sasaki, J., and Taniguchi, K.
Virus Res. 147 ( 1 ) 77 - 84 2010
Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER Joint Work
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Solution structures of the trihelix DNA-binding domains of the wild-type and a phosphomimetic mutant of Arabidopsis GT-1, implying a mechanism for an increase in DNA-binding affinity through phosphorylation.
Nagata T, Niyada E, Fujimoto N, Nagasaki Y, Noto K, Miyanori Y, Murata J, Hiratsuka K, Katahira M
Proteins 78 ( 14 ) 3033 - 47 2010 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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植物機能制御剤の探索・創製とその生理学，化学遺伝学への応用―５ プラントアクティベーターによる植物免疫の活性化と化学遺伝学への利用
鳴坂 義弘, 平塚 和之, 能年 義輝
化学と生物 48 ( 10 ) 706 - 712 2010
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：公益社団法人 日本農芸化学会 Joint Work
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Screening and evaluation of plant activators by the bioluminescence reporter system
Kusama Masahiro, Ogura Reiko, Hiratsuka Kazuyuki
Journal of Pesticide Science 34 ( 4 ) 346 - 349 2009
Language：English Publishing type：Research paper (scientific journal) Publisher：Pesticide Science Society of Japan Joint Work
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Peanut stunt virus 2b cistron plays a role in viral local and systemic accumulation and virulence in Nicotiana benthamiana
Netsu, O., Kuwata, S., Hibi, T., Ugaki, M., and Suzuki, M.
Arch. Virol. 153 ( 9 ) 1731–1735 2008
Language：English Publishing type：Research paper (scientific journal) Publisher：SPRINGER Joint Work
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Practical Uses of Arabidopsis Genome Information; Application in Agrochemicals
Hiratsuka Kazuyuki
Plant and Cell Physiology Supplement 2008 ( 0 ) S0065 - S0065 2008 [Reviewed]
Language：The in addition, foreign language Publishing type：Research paper (scientific journal) Publisher：The Japanese Society of Plant Physiologists Single Work
The availability of genome information on defense-related genes in Arabidopsis and the development of reporter gene technologies have provided the means to construct promoter-reporter gene fusions for defense gene expression monitoring system. We have developed luciferase-based bioluminescence reporter system for defense genes and have shown that the system can be applied for screening of chemicals that induce defense responses in plants. The use of transgenic Arabidopsis plants harboring promoter-luciferase fusion genes in multiwell formats turned out to be an ideal approach for high-throughput screening of chemical libraries that requires a small-scale assay system for initial selection of compounds. We will present some of our results on screening and evaluation of plant activators and discuss the problems associated with this technology and future directions.
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stunt virus 2b cistron plays a role in viral local and systemic accumulation and virulence in Nicotiana benthamiana.
平塚 和之
Arch. Virol. 153 1731 - 1735 2008 [Reviewed]
Language：The in addition, foreign language Publishing type：Research paper (scientific journal) Single Work
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Monitoring of <I>Arabidopsis</I> <I>BIK1</I> Promoter by Bioluminescence Reporter Gene
Banzashi Go, Urata Nobuaki, Tanaka Tsuneyuki, Sasano Kanako, Ono Sachiko, Hiratsuka Kazuyuki
Plant and Cell Physiology Supplement 2008 ( 0 ) 0951 - 0951 2008 [Reviewed]
Language：The in addition, foreign language Publishing type：Research paper (scientific journal) Publisher：The Japanese Society of Plant Physiologists Joint Work
To study the regulated expression of genes involved in the disease resistance, we developed an <I>in vivo</I> monitoring system by the bioluminescence reporter gene and have shown that the system is applicable to the screening of chemicals capable of inducing defense responses in plants. Recently, <I>BIK1</I> gene that shows a characteristic feature in expression pattern in response to fungal infection has been isolated. <I>BIK1</I> gene is induced by necrotrophic pathogens but its expression is rather insensitive to treatment by chemical inducers of defense gene expression. Although its involvement in the crosstalk of defense-related signaling pathways has been suggested, regulated expression of the <I>BIK1</I> gene is largely unknown. To investigate the promoter function of the <I>BIK1</I> gene, we constructed transgenic <I>Arabidopsis</I> lines harboring <I>BIK1</I> promoter-firefly luciferase fusion gene. Here we report some results from our investigation of the <I>BIK1</I> promoter function after inoculation with several pathogens and after treatment with chemicals.
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Functional Analysis of RNA Silencing Suppressors by Microprojectile Bombardment
Hayashihara Chieko, Takeda Tomoko, Ogura Rieko, Matsuo Naoko, Hiratsuka Kazuyuki
Plant and Cell Physiology Supplement 2008 ( 0 ) 0757 - 0757 2008 [Reviewed]
Language：The in addition, foreign language Publishing type：Research paper (scientific journal) Publisher：The Japanese Society of Plant Physiologists Joint Work
To evade host defense mechanism, many RNA viruses encode suppressor of RNA silencing. The use of RNA silencing suppressors for the improvement of foreign protein production levels in transgenic plants has been suggested, but the mechanisms involved in the process are not necessarily clear. To evaluate the function of RNA silencing suppressors we tested transient gene expression system by microprojectile bombardment. Bioluminescence reporter genes fused to the <I>Cauliflower mosaic virus</I> (CaMV) 35S promoter were co-introduced into plant cells with silencing suppressors from <I>Cucumber mosaic virus</I>-encoded 2b or <I>Peanut clump virus</I>-encoded P15. Monitoring of luminescence levels by cooled CCD camera after introduction of expression vectors suggested that we could observe the enhanced expression levels of reporter genes by co-expression of silencing suppressors in the transient assay system.
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Seo, S; Maeda, T; Hiratsuka, K
PLANT BIOTECHNOLOGY 24 ( 3 ) 321 - 329 2007.6
DOI Web of Science CiNii Research
Language：English Publishing type：Research paper (scientific journal) Publisher：日本植物バイオテクノロジー学会 Joint Work
As is the case with other organisms, plants respond to genotoxic stresses by expressing DNA repair genes upon DNA damage. To uncover the mechanisms involved in regulated expression of the DNA damage-responsive gene, we investigated the tissue specific expression and DNA damage-responsiveness of the <i>Arabidopsis RAD51</i> (<i>AtRAD51</i>) gene promoter in <i>Arabidopsis</i> and tobacco. Transgenic <i>Arabidopsis</i> and tobacco plants harboring <i>AtRAD51</i> promoter- β-glucuronidase (GUS) were used to study the detailed expression pattern of the <i>AtRAD51</i> gene. A histochemical GUS assay of bleomycin- or UV-treated plants showed that the <i>AtRAD51</i> promoter in young tissues is actively expressed particularly in meristematic cells of the root and shoot apex of seedlings. In the absence of genotoxic stress, GUS activities were detected only at very low levels in these same organs. In mature plants, the <i>AtRAD51</i> promoter is mainly expressed in flower bud, sepal, stigma, later anther, pedicel when treating with DNA damaging agent. The expression patterns of reporter assays were consistent with the <i>AtRAD51</i> mRNA accumulation pattern. These results suggest that the regulated expression of the <i>AtRAD51</i> gene is controlled mainly at the level of transcription directed primarily by the promoter function of the gene.
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Development of defense gene expression monitoring systems by the bioluminescence reporter genes in higher plants.
XVI International Plant Protection Congress 2007, Congress Proceedings 2 722 - 723 2007 [Reviewed]
Language：English Publishing type：Research paper (international conference proceedings) Joint Work
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Expression and subcellular localization of pre-rRNA processing factor homologues in higher plants.
Plant Biotechnol. 24 301 - 306 2007 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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Tissue-specific and DNA damage-responsive expression of the AtRAD51 gene promoter in transgenic Arabidopsis and tobacco.
Plant Biotechnol. 24 321 - 329 2007 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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Interactions with RNA/DNA of proteins involved in the regulation of transcription, translation and telomere elongation.
Nucleic Acids Symposium 51 77 - 78 2007 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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Development of a high throughput system to screen for candidate plant activators using an immune-induction system in Arabidopsis
NARUSAKA Yoshihiro,HIRATSUKA Kazuyuki,ABE Hiroshi
Plant protection 61 ( 10 ) 537 - 541 2007 [Reviewed]
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japan Plant Protection Association Joint Work
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Seo, S. and Maeda, T. and Hiratsuka, K.
Plant Biotechnology 24 ( 3 ) 321 - 329 2007 [Reviewed]
Language：The in addition, foreign language Publishing type：Research paper (scientific journal) Joint Work
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A high-throughput evaluation system for Arabidopsis mutants for defense signaling.
Plant Biotechnology 23 459 - 466 2006 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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A high-throughput evaluation system for Arabidopsis mutants for defense signaling.
Plant Biotechnol. 23 459 - 466 2006 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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Bioluminescence reporter assay system to monitor Arabidopsis MPK3 gene expression in response to infection by Botrytis cinerea.
J. Gen. Plant Pathol. 72 1 - 5 2006 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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A new species of Pucciniastrum on Enkianthus campanulatus from Japan.
Mycotaxon 92 371 - 376 2005 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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Multi-color luciferases as reporters for monitoring transient gene expression in higher plants.
Plant Biotechnology 22 151 - 155 2005 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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Development of defense gene expression monitoring system and its applications.
Hiratsuka, K; Ono, S; Tanaka, T; Nishiyama, Y; Wako, N
PLANT AND CELL PHYSIOLOGY 46 S13 - S13 2005 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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Characterization of the EMCV-IRES mediated bicistronic translation in plant cells.
Plant Biotechnology 21 ( 240 ) 119 - 126 2004 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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Expression of the AtRAD51 gene promoter in response to DNA damage in transgenic tobacco.
Plant Biotechnology 21 ( 240 ) 113 - 118 2004 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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Transient assay system for the analysis of PR-1a gene promoter in tobacco BY-2 cells.
Biosci. Biotechnol. Biochem. 68 ( 4 ) 803 - 807 2004 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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Transient assay system for the analysis of PR-lα gene promoter in tobacco BY-2 cells.
平塚 和之
Biosci.Biotechnol.Biochcm. 68 803 - 807 2004 [Reviewed]
Language：The in addition, foreign language Publishing type：Research paper (scientific journal) Single Work
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Transient assay system for the analysis of PR-la gene promoter in tobacco BY-2 cells.
平塚 和之
Biosci.Biotechnol.Biochem. 68 803 - 807 2004 [Reviewed]
Language：The in addition, foreign language Publishing type：Research paper (scientific journal) Single Work
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Identification of novel microsporogenesis-associated genes encoding proteins with a nuclear localization signal.
Plant Biotechnology 20 137 - 143 2003 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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Isolation and characterization of a novel GRAS gene that regulates meiosis-associated gene expression.
J. Biol. Chem. 278 20865 - 20873 2003 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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Assessment of utility of meiosis-associated promoters of lily for induction of germinal ds transposition in transgenic rice.
Plant Cell Physiol. 44 637 - 642 2003 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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Induction of Ds transposition using meiosis-associated promoters in transgenic rice
MORITA R., YOKOI S., TAKASE N., HIRATSUKA K., TORIYAMA K.
4 194 2002.8 [Reviewed]
Language：Japanese Publishing type：Research paper (scientific journal) Joint Work
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Characterization of a Novel GT-box Binding Protein from Arabidopsis.
MURATA Jun, TAKASE Hisabumi, HIRATSUKA Kazuyuki
Ｐｌａｎｔ Ｂｉｏｔｅｃｈｎｏｌｏｇｙ 19 ( 2 ) 103 - 112 2002
Language：English Publishing type：Research paper (scientific journal) Publisher：日本植物バイオテクノロジー学会 Joint Work
We isolated a cDNA encoding a novel GT-box binding protein from Arabidopsis, designated GT-4. The predicted open reading frame encodes a protein of 372 amino acids and the predicted protein sequence revealed the presence of a putative DNA-binding domain with 80% homology to the trihelix region of previously described light-responsive element binding protein, GT-1. Reverse transcription-PCR analysis showed that GT-4 transcripts are present in all tissues tested in light-grown plants, but light-regulated expression of GT-4 mRNA was observed only in etiolated seedlings. Electro-mobility shift assay using recombinant protein revealed that the GT-box-binding specificity of GT-4 is almost identical to that of GT-1. Transient expression of GT-4::GFP fusion protein in onion epidermal cells revealed the presence of a nuclear localization signal within the GT-4 protein. These results suggest the possibility that GT-4 is involved in GT-box-mediated gene expression by recognizing target sequences closely related to GT-1 binding sites.
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Modulation of GT-1 DNA-binding activity by calcium-dependent phosphorylation
Maréchal, E; Hiratsuka, K; Delgado, J; Nairn, A; Qin, J; Chait, BT; Chua, NH
PLANT MOLECULAR BIOLOGY 40 ( 3 ) 373 - 386 1999.6 [Reviewed]
DOI Web of Science Scopus PubMed
Language：The in addition, foreign language Publishing type：Research paper (scientific journal) Joint Work
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Function of proteins involving the chromosome construction during meiosis
Hotta, Y. and Hiratsuka, K. and Takase, H.
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme 44 ( 12 Suppl ) 1741 - 1748 1999 [Reviewed]
Language：The in addition, foreign language Publishing type：Research paper (scientific journal) Joint Work
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Germ cell formation and gametophyte differentiation in higher plants
Hiratsuka, K. and Takase, H. and Hotta, Y.
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme 43 ( 4 ) 602 - 608 1998 [Reviewed]
Language：The in addition, foreign language Publishing type：Research paper (scientific journal) Joint Work
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Light regulated transcription in higher plants
Hiratsuka, K. and Chua, N.-H.
Journal of Plant Research 110 ( 1097 ) 131 - 139 1997 [Reviewed]
Language：The in addition, foreign language Publishing type：Research paper (scientific journal) Joint Work
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Phytochrome-regulated repression of gene expression requires calcium and cGMP
Neuhaus, G. and Bowler, C. and Hiratsuka, K. and Yamagata, H. and Chua, N.-H.
EMBO Journal 16 ( 10 ) 2554 - 2564 1997 [Reviewed]
Language：The in addition, foreign language Publishing type：Research paper (scientific journal) Joint Work
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Calcium and cGMP target distinct phytochrome-responsive elements
Wu, Y. and Hiratsuka, K. and Neuhaus, G. and Chua, N.-H.
Plant Journal 10 ( 6 ) 1149 - 1154 1996 [Reviewed]
Language：The in addition, foreign language Publishing type：Research paper (scientific journal) Joint Work
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KAGAKU TO SEIBUTSU 34 ( 10 ) 683 - 691 1996
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japan Society for Bioscience, Biotechnology, and Agrochemistry Joint Work
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光による遺伝子発現制御
平塚 和之
日本植物学会大会研究発表記録 = Proceedings of the annual meeting of the Botanical Society of Japan 59 57 1995.9 [Reviewed]
Language：Japanese Publishing type：Research paper (scientific journal) Single Work
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Transcription factors in plant growth and development
Ramachandran, S. and Hiratsuka, K. and Chua, N.-H.
Current Opinion in Genetics and Development 4 ( 5 ) 642 - 646 1994 [Reviewed]
Language：The in addition, foreign language Publishing type：Research paper (scientific journal) Joint Work
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Gilmartin, P.M. and Memelink, J. and Hiratsuka, K. and Kay, S.A. and Chua, N.-H.
Plant Cell 4 ( 7 ) 839 - 849 1992 [Reviewed]
Language：The in addition, foreign language Publishing type：Research paper (scientific journal) Joint Work
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Nucleotide Sequence of the 3'-Terminal Region of Potato Virus T RNA.
OCHI Motoyasu, KASHIWAZAKI Satoshi, HIRATSUKA Kazuyuki, NAMBA Shigetou, TSUCHIZAKI Tsuneo
Japanese Journal of Phytopathology 58 ( 3 ) 416 - 425 1992
Language：English Publishing type：Research paper (scientific journal) Publisher：The Phytopathological Society of Japan Joint Work
The partial sequence of the capillovirus potato virus T (PVT) RNA containing the 3′-terminal 2392 nucleotides, excluding the poly (A) tail, was determined from cloned cDNA. The sequence contains three open reading frames (ORFs) which encode putative proteins (in the 5′→3′ direction) of <i>M<sub>r</sub></i>>29, 000 (>29K; ORF 1), <i>M<sub>r</sub></i> 40, 451 (40K; ORF 2) and <i>M<sub>r</sub></i> 23, 596 (24K; ORF 3), followed by an untranslated region of 188 nucleotides upstream of the 3′ poly (A) tail. The 5′-proximal ORF 1 encodes a product with significant homologies to the C-terminal portions of the putative polymerase proteins of “alpha-like” supergroup of plant RNA viruses, including apple chlorotic leaf spot virus (ACLSV; closterovirus subgroup A), carlavirus, potexvirus and tymovirus. The PVT 24K protein shared several blocks of conserved amino acids with the coat proteins of filamentous viruses, <i>i.e.</i> ACLSV, beet yellows virus (BYV; closterovirus subgroup B), citrus tristeza virus (CTV; closterovirus subgroup C), potexvirus and carlavirus. The PVT 40K protein had homologies with putative movement proteins encoded by several plant viruses (e.g. ACLSV 50K protein). PVT and ACLSV showed a similar arrangement of three ORFs in the 3′-terminal region of their genomes. However, this arrangement differs significantly from that of BYV. The sequence represented here is the first report on the group capillovirus which was established recently. These results support a close relationship of PVT with ACLSV which is currently classified in the closterovirus subgroup A.
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Firefly luciferase as a reporter of regulated gene expression in higher plants
Millar, A.J. and Short, S.R. and Hiratsuka, K. and Chua, N.-H. and Kay, S.A.
Plant Molecular Biology Reporter 10 ( 4 ) 324 - 337 1992 [Reviewed]
Language：The in addition, foreign language Publishing type：Research paper (scientific journal) Joint Work