Review Papers - KOJIMA Chojiro
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オルガネラ間コンタクトを担うVAP蛋白質の構造と機能
古板恭子, 児嶋長次郎
酵素工学ニュース Vol.81 12 - 16 2019.4
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution) Joint Work
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Toshihiko Sugiki and Kyoko Furuita and Toshimichi Fujiwara and Chojiro Kojima
Biochemistry 57 ( 26 ) 3576 - 3589 2018.7
Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：American Chemical Society ({ACS}) Single Work
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Current NMR Techniques for Structure-Based Drug Discovery
Toshihiko Sugiki, Kyoko Furuita, Toshimichi Fujiwara, and Chojiro Kojima
Molecules 23 ( 1 ) 148 2018.1 [Reviewed] [Invited]
Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：MDPI Joint Work
A variety of nuclear magnetic resonance (NMR) applications have been developed for structure-based drug discovery (SBDD). NMR provides many advantages over other methods, such as the ability to directly observe chemical compounds and target biomolecules, and to be used for ligand-based and protein-based approaches. NMR can also provide important information about the interactions in a protein-ligand complex, such as structure, dynamics, and affinity, even when the interaction is too weak to be detected by ELISA or fluorescence resonance energy transfer (FRET)-based high-throughput screening (HTS) or to be crystalized. In this study, we reviewed current NMR techniques. We focused on recent progress in NMR measurement and sample preparation techniques that have expanded the potential of NMR-based SBDD, such as fluorine NMR (19F-NMR) screening, structure modeling of weak complexes, and site-specific isotope labeling of challenging targets.A variety of nuclear magnetic resonance (NMR) applications have been developed for structure-based drug discovery (SBDD). NMR provides many advantages over other methods, such as the ability to directly observe chemical compounds and target biomolecules, and to be used for ligand-based and protein-based approaches. NMR can also provide important information about the interactions in a protein-ligand complex, such as structure, dynamics, and affinity, even when the interaction is too weak to be detected by ELISA or fluorescence resonance energy transfer (FRET)-based high-throughput screening (HTS) or to be crystalized. In this study, we reviewed current NMR techniques. We focused on recent progress in NMR measurement and sample preparation techniques that have expanded the potential of NMR-based SBDD, such as fluorine NMR (19F-NMR) screening, structure modeling of weak complexes, and site-specific isotope labeling of challenging targets.
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Protein 19F-labeling using transglutaminase for the NMR study of intermolecular interactions
Yoshikazu Hattori and David Heidenreich and Yuki Ono and Toshihiko Sugiki and Kei-ichi Yokoyama and … Show more authors
Yoshikazu Hattori and David Heidenreich and Yuki Ono and Toshihiko Sugiki and Kei-ichi Yokoyama and Ei-ichiro Suzuki and Toshimichi Fujiwara and Chojiro Kojima Hide authors
Journal of Biomolecular NMR 68 ( 4 ) 271 - 279 2017.8
Language：The in addition, foreign language Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution) Publisher：Springer Science and Business Media {LLC} Single Work
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Lysine <sup>13</sup>C-Methylation NMR for Analyses of Interactions and Structural Changes
HATTORI Yoshikazu,KOJIMA Chojiro
Journal of the Biophysical Society of Japan 56 ( 5 ) 288 - 289 2016.9 [Reviewed] [Invited]
Language：Japanese Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：The Biophysical Society of Japan Joint Work
Other Link： https://www.jstage.jst.go.jp/article/biophys/56/5/56_288/_article/-char/ja/
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Latest approaches for efficient protein production in drug discovery
Toshihiko Sugiki, Toshimichi Fujiwara, and Chojiro Kojima
Expert Opin. Drug Discov. 9 ( 10 ) 1189 - 1204 2014.10 [Reviewed] [Invited]
Language：English Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Joint Work