Papers - KURIHARA Yasuyuki
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Atsumi Sakaguchi, Yoichiro Tanaka, Eiki Shoji, Teppei Takeshima, Rina Sakamaki, Takao Matsuba and … Show more authors
Atsumi Sakaguchi, Yoichiro Tanaka, Eiki Shoji, Teppei Takeshima, Rina Sakamaki, Takao Matsuba and Yasuyuki Kurihara Hide authors
Journal of Biological Engineering 17 ( 1 ) 2023.3 [Reviewed]
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Nature Joint Work
BACKGROUND: Monoclonal antibodies are essential in life science research and developing antibody drugs and test drugs. Various methods have been developed to obtain monoclonal antibodies, among which hybridoma technology continues to be widely used. However, developing a rapid and efficient method for obtaining conformation-specific antibodies using hybridoma technology remains challenging. We previously developed the membrane-type immunoglobulin-directed hybridoma screening (MIHS) method, which is a flow cytometry-based screening technique based on the interaction between the B-cell receptor expressed on the hybridoma cell surface and the antigen protein, to obtain conformation-specific antibodies.
RESULTS: In this study, we proposed a streptavidin-anchored ELISA screening technology (SAST) as a secondary screening method that retains the advantages of the MIHS method. Anti-enhanced green fluorescent protein monoclonal antibodies were generated as a model experiment, and their structural recognition abilities were examined. Examination of the reaction profiles showed that all monoclonal antibodies obtained in this study recognize the conformational epitopes of the protein antigen. Furthermore, these monoclonal antibodies were classified into two groups: those with binding activities against partially denatured proteins and those with complete loss of binding activities. Next, when screening monoclonal antibodies by the MIHS method as the first screening, we found that monoclonal antibodies with stronger binding constants may be selected by double-staining for hybridomas with fluorescently labeled target antigens and fluorescently labeled B cell receptor antibodies.
CONCLUSIONS: The proposed two-step screening method, which incorporates MIHS and SAST, constitutes a rapid, simple, and effective strategy to obtain conformation-specific monoclonal antibodies generated through hybridoma technology. The novel monoclonal antibody screening strategy reported herein could accelerate the development of antibody drugs and antibody tests.Other Link： https://link.springer.com/article/10.1186/s13036-023-00345-9
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Atsumi Sakaguchi, Chika Nakajima, Ayuko Sawano, Yoichiro Tanaka, and Yasuyuki Kurihara
J.Biosci. Bioeng. 131 ( 6 ) 696 - 702 2021.2 [Reviewed]
DOI Web of Science PubMed CiNii Research
Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier Joint Work
Monoclonal antibodies are extremely valuable functional biomaterials that are widely used not only in life science research but also in antibody drugs and test drugs. There is also a strong need to develop high-quality neutralizing antibodies as soon as possible in order to stop the rapid spread of new infectious diseases such as the SARS-CoV-2 virus. This study has developed a membrane-type immunoglobulin-directed hybridoma screening (MIHS) method for obtaining high-quality monoclonal antibodies with high efficiency and high speed. In addition to these advantages, this paper demonstrates that the MIHS method can selectively obtain monoclonal antibodies that specifically recognize the functional structure of proteins. The MIHS method is a useful technology that greatly contributes to the research community because it can be easily introduced in any laboratory that uses a flow cytometer.
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Masahiro Endou, Kaito Yoshida, Makoto Hirota, Chika Nakajima, Atsumi Sakaguchi, Naoto Komatsubara,Y … Show more authors
Masahiro Endou, Kaito Yoshida, Makoto Hirota, Chika Nakajima, Atsumi Sakaguchi, Naoto Komatsubara,Yasuyuki Kurihara Hide authors
Mitochondrion 52 1 - 7 2020.2 [Reviewed]
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier Joint Work
We identified Coxfa4l3, previously called C15orf48 or Nmes1, as a novel accessory protein of Complex IV of the mitochondrial electron transport chain (ETC). Amino acid sequence comparison, the intracellular localization and the protein expression data showed that the protein is the third isoform of Coxfa4 and the expression of Coxfa4 and Coxfa4l3 proteins during spermatogenesis showed a mutually exclusive pattern, implying that Coxfa4 replaces Coxfa4l3 in Complex IV after meiosis. These results may provide some insight into the unique mechanism of ATP production in late spermatogenesis.
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Flow cytometry-based method for rapid and high-throughput screening of hybridoma cells secreting monoclonal antibody
赤城幸、中嶋千佳、田中陽一郎、栗原靖之
Journal of Bioscience and Bioengeering 125 ( 4 ) 464 - 469 2018.5 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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Ectopic expression of the mitochondrial protein COXFA4L3 in human sperm acrosome and its potential application in the selection of male infertility treatments
Yusuke Fujisawa, Sayaka Kikuchi, Fujino Kuba, Kosei Oishi, Soushi Murayama, Tomoya Sugiyama, Reiji … Show more authors
Yusuke Fujisawa, Sayaka Kikuchi, Fujino Kuba, Kosei Oishi, Soushi Murayama, Tomoya Sugiyama, Reiji Tokito, Hiroe Ueno, Shin-ichi Kashiwabara, Yasushi Yumura, and Yasuyuki Kurihara Hide authors
Reproductive Medicine and Biology 2024 [Reviewed]
Authorship：Last author, Corresponding author Language：English Publishing type：Research paper (scientific journal) Single Work
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Monoclonal antibody production technology optimised for cytometry: from theory to practice.
Sakaguchi Atsumi, Kurihara Yasuyuki
Cytometry Research 34 ( 1 ) 9 - 15 2024.9 [Reviewed]
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japan Cytometry Society Single Work
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Dynamic paraspeckle component localization during spermatogenesis
Andrew T Major , Cathryn A Hogarth , Julia C Young, Yasuyuki Kurihara, David A Jans and Kate L Love … Show more authors
Andrew T Major , Cathryn A Hogarth , Julia C Young, Yasuyuki Kurihara, David A Jans and Kate L Loveland Hide authors
Reproduction Vol.153 ( 3 ) 267 - 280 2019.9 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Publisher：Bioscientifica Ltd Joint Work
Other Link： https://rep.bioscientifica.com/view/journals/rep/158/3/REP-19-0139.xml
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Specific intron-dependent loading of DAZAP1 onto the cox6c transcript suppresses pre-mRNA splicing efficacy and induces cell growth retardation
Kenta Sasaki, Mana Ono, Kaori Takabe, Atsushi Suzuki, Yasuyuki Kurihara
GENE 2018 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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Specific interaction with the nuclear transporter importin a2 can modulate paraspeckle protein 1 delivery to nuclear paraspeckles.
Andrew T. Major, Cathryn A. Hogarth, Yoichi Miyamoto, Mai A. Sarraj, Catherine L. Smith f, Peter Ko … Show more authors
Andrew T. Major, Cathryn A. Hogarth, Yoichi Miyamoto, Mai A. Sarraj, Catherine L. Smith f, Peter Koopman, Yasuyuki Kurihara, David A. Jans and Kate L. Loveland Hide authors
Molecular Biology of the Cell 26 ( 8 ) 1543 - 1548 2015.4 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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Mitochondrial Biogenesis and the Activity Regulation by Posttranscriptional Control of cox6c mRNA by an RNA Binding Protein, Prrp
Kurihara Y, Sakagami H, Ono M, Takabe K, Sasaki K, Tasaka Y, Yanai C and Yasuhara A
International Symposium on Mitochondria 2014 2014.11 [Reviewed]
Language：English Publishing type：Research paper (international conference proceedings) Joint Work
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Distinct roles of DBHS family members in the circadian transcriptional feedback loop.
Elzbieta Kowalska, Juergen Ripperger, Christine Muheim, Bert Maier, Yasuyuki Kurihara, Archa Fox, A … Show more authors
Elzbieta Kowalska, Juergen Ripperger, Christine Muheim, Bert Maier, Yasuyuki Kurihara, Archa Fox, Achim Kramer, and Steven A. Brown Hide authors
Molecular and Cellular Biology 32 ( 22 ) 4585 - 4594 2012.11 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Publisher：AMER SOC MICROBIOLOGY Joint Work
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Acetylation of Prrp K150 regulates the subcellular localization.
Kenta SASAKI, Atsushi SUZUKI, Satoshi KAGATSUME, Katsuhiko MATSUZAWA, Mana Ono, Yusuke TAGUCHI, Yas … Show more authors
Kenta SASAKI, Atsushi SUZUKI, Satoshi KAGATSUME, Katsuhiko MATSUZAWA, Mana Ono, Yusuke TAGUCHI, Yasuyuki KURIHARA Hide authors
GENE 491 ( 1 ) 13 - 19 2012.1 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER Joint Work
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Specific interaction of the nuclear transporter importin alpha 2 with paraspeckle protein 1 modulates nuclear paraspeckle formation
A Major, Hogarth CA, Miyamoto Y, Sarraj MA, Smith CL, Koopman P, Kurihara Y, Jans DA, Loveland KA
The 12th International Symposium on Spermatology 2011.8 [Reviewed]
Language：English Publishing type：Research paper (international conference proceedings) Joint Work
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Maintenance of Dendritic Spine Morphology by Partitioning-Defective 1b through Regulation of Microtubule Growth.
Hayashi K, Suzuki A, Hirai S, Kurihara Y, Hoogenraad CC, Ohno S.
J Neurosci. 31 ( 34 ) 12094 - 12103 2011.8 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Publisher：SOC NEUROSCIENCE Joint Work
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Mechanism of germ cell development Toward assisted new reproductive technologies
Suzuki A., Kurihara Y., Saga Y
Reproductive Medicine and Biology 2011
Language：Japanese Publishing type：Research paper (scientific journal) Joint Work
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Bovine Sertoli Cells Colonize and Form Tubules in Murine Hosts Following Transplantation and Grafting Procedures.
Zhang Z, Hill J, Holland M, Kurihara Y, Loveland KL
Journal of Andrology 29 ( 4 ) 418 - 430 2008 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Publisher：AMER SOC ANDROLOGY Joint Work
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Structure, interactions and effects on activity of the 5'-terminal region of human telomerase RNA
Li, Xianglan; Nishizuka, Hidetoshi; Tsutsumi, Kota; Imai, Yuka; Kurihara, Yasuyuki; Uesugi, Seiichi
Journal of Biochemistry 141 ( 5 ) 755 - 765 2007.5 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE BIOCHEMICAL SOC Joint Work
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PSPC1, NONO, and SFPQ Are Expressed in Mouse Sertoli Cells and May Function as Coregulators of Androgen Receptor-Mediated Transcription.
Kuwahara S, Ikei A, Taguchi Y, Tabuchi Y, Fujimoto N, Obinata M, Uesugi S, Kurihara Y.
Biology of Reproduction 75 ( 3 ) 352 - 359 2006.4 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Publisher：SOC STUDY REPRODUCTION Joint Work
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Enhanced gene targeting efficiency by siRNA that silences the expression of the Bloom syndrome gene in human cells.
So S, Nomura Y, Adachi N, Kobayashi Y, Hori T, Kurihara Y, Koyama H.
Genes To Cells 11 ( 4 ) 363 - 371 2006.4 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Publisher：BLACKWELL PUBLISHING Joint Work
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The RNA ligands for mouse proline-rich RNA-binding protein (mouse Prrp) contain two consensus sequences in separate loop structure.
Hori T, Taguchi Y, Uesugi S, Kurihara Y
Nucleic Acids Research 33 ( 1 ) 190 - 200 2005.1 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Publisher：OXFORD UNIV PRESS Joint Work
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Dynamic changes of intranuclear and subcellular localizations of mouse Prrp/DAZAP1 during spermatogenesis and the C-terminal proline-rich region is required for nuclear import and nuclear localization.
Kurihara Y, Watanabe H, Kawaguchi A, Hori T, Mishiro K, Ono M, Sawada H and Uesugi S
Arch. Histol. Cytol. 67 ( 4 ) 325 - 333 2004.12 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Publisher：INT SOC HISTOLOGY & CYTOLOGY Joint Work
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Expression and functional significance of mouse paraspeckle protein 1 (mPSP1) on spermatogenesis.
Myojin R, Kuwahara S, Yasaki T, Matsunaga T, Sakurai T, Kimura M, Uesugi S and Kurihara Y
Biology of Reproduction 71 ( 3 ) 926 - 932 2004 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Publisher：SOC STUDY REPRODUCTION Joint Work
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Identification and characterization of CPEB2, a novel putative translational regulator in mouse haploid germ cells.
Biology of Reproduction 69 261 - 268 2003 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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Cleavage reaction of HDV ribozymes in the presence of Mg2+ is accompanied by a conformational change.
Tanaka Y, Tagaya M, Hori T, Sakamoto T, Kurihara Y, Katahira M, Uesugi S.
Genes To Cells 7 567 - 579 2002.6 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Publisher：BLACKWELL PUBLISHING LTD Joint Work
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Imino proton NMR analysis of HDV ribozymes: nested double psuedoknot structure and Mg2+ ion-binding site close to the catalytic core in solution.
Nuc. Acids Res 30 766 - 774 2002 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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Altering the GTP binding site of the DNA/RNA binding protein, Translin/TB-RBP, decreases RNA binding and may create a dominant negative phenotype
V. M. Chennathukuzhi, Y. Kurihara, J. D. Bray, J. Yang and N. Hecht.
Nucleic Acids Res. 26 ( 21 ) 4433 - 4440 2001.11 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Publisher：OXFORD UNIV PRESS Joint Work
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Polypeptide synthesis using an expressed peptide as a building block for condensation with a peptide thioester: application to the synthesis of phosphorylated p21Max protein (1-101)
Kawakami T, Hasegawa K, Teruya K, Akaji K, Horiuchi M, Inagaki F, Kurihara Y, Uesugi S, Aimoto S.,
J. Pept. Sci., 7 ( 9 ) 474 - 487 2001.9 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Publisher：JOHN WILEY & SONS LTD Joint Work
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Trax (Translin associated factor X), a Primarily Cytoplasmic Protein, Inhibits the Binding of TB-RBP (Translin)to RNA.
V. M. Chennathukuzhi, Y. Kurihara, J. D. Bray and N. Hecht,
J. Biol. Chem 276 ( 16 ) 13256 - 13263 2001.4 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Publisher：AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC Joint Work
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Polypeptide synthesis using expressed peptide as a building block via the thioester method.
T. Kawakami, K. Hasegawa, K. Teruya, K. Akaji, M. Horiuchi, F. Inagaki, Y. Kurihara, S. Uesugi and … Show more authors
T. Kawakami, K. Hasegawa, K. Teruya, K. Akaji, M. Horiuchi, F. Inagaki, Y. Kurihara, S. Uesugi and S. Aimoto. Hide authors
Tetrahedron Let. 41 ( 15 ) 2625 - 2628 2000.4 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER Joint Work
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Structure, backborndynamics and interactions with RNA of the C-termininal RNA-binding domain of a mouse neural RNA-binding protein, Musashi1.
J. Mol. Biol. 287 314 - 330 1999 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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Structure and interactions with RNA of the N-terminal UUAG-specific RNA-binding domain of hnRNPD0.
J. Mol. Biol. 287 221 - 237 1999 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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Structural investigation of HDV ribizymes by NMR spectroscopy.
Nucleic Acids Symposium Series 38 147 - 148 1998 [Reviewed]
Language：English Publishing type：Research paper (international conference proceedings) Joint Work
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Dental caries susceptibility in mice is closely linked to the H-2 region on chromosome 17.
N. Suzuki, Y. Kurihara and Y. Kurihara
Carious Res. 32 ( 4 ) 262 - 265 1998 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Publisher：KARGER Joint Work
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Isolation of a sex chromosome-specific DNA sequence in the medaka, Oryzias latipes.
M. Matsuda, T. Kusama, T. Oshiro, Y. Kurihara, S. Hamaguchi and M. Sakaizumi
Genes & Genetic Systems 72 ( 5 ) 263 - 268 1997.10 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Publisher：GENETICS SOC JAPAN Joint Work
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Properties of Hepatitis Delta Virus ribozyme, which consists of three RNA oligomer strands.
T. Sakamoto, Y. Tanaka, T. Kuwabara, M. Kim, Y. Kurihara, M. Katahira and S. Uesugi
J. Biochem. 121 ( 6 ) 1123 - 1128 1997.6 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE BIOCHEMICAL SOC Joint Work
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Structural properties and RNA-binding activities of two RNA-Recognition Motifs of a neural RNA binding protein, mouse-Musashi-1.
Gene 186 ( 1 ) 21 - 27 1997.2 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER Joint Work
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Properties of a hammerhead ribozyme with deletion of the stem II.
J. Biochem. 121 ( 2 ) 288 - 294 1997.2 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE BIOCHEMICAL SOC Joint Work
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Structure-activity correlation for an HDV ribozyme composed of three RNA strands.
Nucleic Acids Symposium Series 37 309 - 310 1997 [Reviewed]
Language：English Publishing type：Research paper (international conference proceedings) Joint Work
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Dimerization and DNA binding facilitate α-Helix formation of Max in solution.
J. Biochem. 122 711 - 716 1997 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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Hairpin structure of an RNA 28-mer, which contains a sequence of the enzyme component of a hammerhead ribozyme system: evidence for tandem G:A pairs that are not of side-by-side type.
J.Biochem. 122 556 - 562 1997 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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Mouse-Musashi-1, a neural RNA-binding protein highly enriched in the mammalian CNS stem cell
S. Sakakibara, T. Imai, K. Hamaguchi, M. Okabe, J. Aruga,K. Nakajima, D. Yasutomi, T. Nagata, Y. Ku … Show more authors
S. Sakakibara, T. Imai, K. Hamaguchi, M. Okabe, J. Aruga,K. Nakajima, D. Yasutomi, T. Nagata, Y. Kurihara, S. Uesugi, T. Miyata, M. Ogawa, K. Mikoshiba, and H. Okano Hide authors
Developmental Biology 176 ( 2 ) 230 - 242 1996.6 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Publisher：ACADEMIC PRESS INC Joint Work
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Effects of metal ions on activity and structure of a lead ribozyme.
Nucleic Acids Symposium Series 35 133 - 134 1996 [Reviewed]
Language：English Publishing type：Research paper (international conference proceedings) Joint Work
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Characterization of an HDV ribozyme which consists of three RNA oligomer strands
T. Sakamoto, T. Kuwabara, Y. Tanaka, Y. Kurihara, M. Katahira and S. Uesugi
Nucleic Acids Symposium Series 32 131 - 132 1995
Language：English Publishing type：Research paper (scientific journal) Single Work
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Structure of translation initiation region of c-myc mRNA and thermostability of the c-myc mRNA/antisense ODN complex.
T. Tanaka, Y. Kurihara, T. Sakamoto, M. Furusawa, A. Kobayashi, M. Katahira, K. Takeuchi, N. Sugimo … Show more authors
T. Tanaka, Y. Kurihara, T. Sakamoto, M. Furusawa, A. Kobayashi, M. Katahira, K. Takeuchi, N. Sugimoto and S. Uesugi Hide authors
Nucleic Acids Symposium Series 32 135 - 136 1995 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Single Work
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Chromosomal locations of Ag-NORs and clusters of ribosomal DNA in laboratory strains of mice.
Y. Kurihara, D. S. Suh, H. Suzuki, and K. Moriwaki(
Mammalian Genome 5 225 - 228 1994 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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Characterization of a hammerhead ribozyme with deletion of the stem II.
T. Sakamoto, M. Kim, Y. Kurihara, N. Sasaki, T. Noguchi, M. Katahira and S. Uesugi
Nucleic Acids Symposium Series 31 189 - 190 1994 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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DNA binding properties of c-Myc related bHLH/LZ oncoproteins.
Y. Kurihara, M. Horiuchi, S. Ukita, M. Katahira, and S. Uesugi
Nucleic Acids Symposium Series 29 169170 1993 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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Cytogenetic adaptive response to low-dose of ionizing radiation in fish cells.
Y. Kurihara, M. Sakaizumi, and Y. Hyodo-Taguchi
The Fish Biology Journal Medaka 1992 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Single Work
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Caries susceptibility in inbred mouse strains and inheritance pattern in F1 and backcross(N2) progeny from strains with high and low caries susceptibility.
Y. Kurihara, T. Naito, K.Obayashi, M. Hirasawa, Y. Kurihara, and K. Moriwaki
Caries Research 25 341 - 346 1991 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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Variation in the distribution of silver staining nucleolar organizer regions on the chromosomes of the wild mice, Mus musculus.
H. Suzuki, Y. Kurihara, T. Kanehisa, and K. Moriwaki
Molecular Biology and Evolution 7 271 - 282 1990 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Single Work
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Ly-2.3 antigen derived from subspecies of the Asian mouse (Mus musculus castaneus).
Y. Kurihara, M. Sakaizumi, Yong Hoi-Sen, T. Kanehisa, and K. Moriwaki
Immunogenetics 28 289 - 291 1988 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work
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Serological Survey of T-lymphocyte Differentiation Antigens in Wild Mice.
Y. Kurihara, N. Miyashita, K. Moriwaki, M. L. Petras, F. Bonhomme, W. S. Cho, and S. Kohno
Immunogenetics 22 211 - 218 1985 [Reviewed]
Language：English Publishing type：Research paper (scientific journal) Joint Work