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• Rejuvenation Of Human Adult Dermal Papilla Cells By Partial Reprogramming For Hair Regenerative Medicine

  Nanmo, A; Suzuki, A; Kageyama, T; Fukuda, J

  TISSUE ENGINEERING PART A   30 ( 15-16 )   S283 - S284   2024.8  [Reviewed]

  Web of Science

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  Authorship：Lead author   Language：English   Publishing type：Research paper (international conference proceedings)   Joint Work  

• Large-Scale Preparation of Hair Follicle Germs Using a Microfluidic Device

  Sugiyama Ellen, Nanmo Ayaka, Nie Xiaolei, Chang Shu-Yung, Hashimoto Michinao, Suzuki Atsushi, Kagey … Show more authors

  ACS Biomaterials Science & Engineering   10 ( 2 )   998 - 1005   2024.1  [Reviewed]

  DOI Web of Science PubMed CiNii Research

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  Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society   Joint Work  

  Hair follicle morphogenesis during embryonic development is driven by the formation of hair follicle germs (HFGs) via interactions between epithelial and mesenchymal cells. Bioengineered HFGs are potential tissue grafts for hair regenerative medicine because they can replicate interactions and hair follicle morphogenesis after transplantation. However, a mass preparation approach for HFGs is necessary for clinical applications, given that thousands of de novo hair follicles are required to improve the appearance of a single patient with alopecia. In this study, we developed a microfluidics-based approach for the large-scale preparation of HFGs. A simple flow-focusing microfluidic device allowed collagen solutions containing epithelial and mesenchymal cells to flow and generate collagen microbeads with distinct Janus structures. During the 3 days of culture, the collagen beads contracted owing to cellular traction forces, resulting in collagen- and cell-dense HFGs. The transplantation of HFGs into nude mice resulted in highly efficient de novo hair follicle regeneration. This method provides a scalable and robust tissue graft preparation approach for hair regeneration.

• Bioprinting of hair follicle germs for hair regenerative medicine *

  Nanmo Ayaka, Yan Lei, Asaba Tomoki, Wan Licheng, Kageyama Tatsuto, Fukuda Junji

  Acta Biomaterialia   165   50 - 59   2023.6  [Reviewed]

  DOI Web of Science PubMed CiNii Research

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  Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier Science Ltd.   Joint Work  

  Hair regenerative medicine is a promising approach to treat hair loss. The replication of in vivo tissue con-figurations and microenvironments, such as hair follicle germs, has been studied to prepare tissue grafts for hair regenerative medicine. However, such approaches should be scalable, because a single patient with alopecia requires thousands of tissue grafts. In this paper, we propose an approach for the scalable and automated preparation of highly hair-inductive tissue grafts using a bioprinter. Two collagen droplets (2 & mu;L each) containing mesenchymal and epithelial cells were placed adjacent to each other to fabricate hair-follicle-germ-like grafts. During three days of culture, the pairs of microgel beads were spontaneously contracted by cell traction forces, whereas the two cell types remained separated, where the densities of the cells and collagen were enriched more than 10 times. This approach allowed us to fabricate submil-limeter objects printed with millimeter-order accuracy, facilitating scalable and automated tissue graft preparation. Because of mesenchymal-epithelial interactions, hair microgels (HMGs, i.e., collagen-and cell-enriched microgels) efficiently regenerate hair follicles and shafts when transplanted into the back skin of mice. However, the generated hair shafts mostly remain under the skin. Therefore, we printed microgel beads onto surgical suture guides arrayed on a stage. The microgel beads were contracted along with the suture guides in culture prior to transplantation. The guide-inserted HMGs significantly im-proved hair-shaft sprouting through the skin, owing to the control of the orientation of the HMGs trans-planted into the skin. This approach is a promising strategy to advance hair regenerative medicine.Statement of significance This study proposes an approach for the scalable and automated preparation of highly hair-inductive grafts using a bioprinter. Two collagen droplets containing mesenchymal and epithelial cells were placed adjacently. Cell traction forces caused the pairs of microgel beads to spontaneously contract in culture. Because of mesenchymal-epithelial interactions, hair microgels (HMGs) efficiently regenerated hair fol-licles on the back skin of mice. However, the generated hair shafts remained mostly beneath the skin. Therefore, we printed microgel beads onto surgical suture guides arrayed on a stage. The guide-inserted HMGs significantly improved hair-shaft sprouting through the skin owing to the control of the orientation of the HMGs in the skin. This approach represents a promising strategy for advancing hair regenerative medicine.& COPY; 2022 The Author(s). Published by Elsevier Ltd on behalf of Acta Materialia Inc. This is an open access article under the CC BY license ( http://creativecommons.org/licenses/by/4.0/ )

• The role of lipids in promoting hair growth through HIF-1 signaling pathway

  Seo, J; Matsumoto, K; Nanmo, A; Tu, S; Jeong, D; Chun, YS; Yan, L; Kageyama, T; Fukuda, J

  SCIENTIFIC REPORTS   15 ( 1 )   2025.2  [Reviewed]

  DOI Web of Science PubMed

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  Language：English   Publishing type：Research paper (scientific journal)   Joint Work  

• Effects of the PI3K/Akt signaling pathway on the hair inductivity of human dermal papilla cells in hair beads

  Seibutsu-kogaku Kaishi   102 ( 2 )   65 - 65   2024.2

  DOI CiNii Research

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  Language：Japanese   Publishing type：Research paper (other science council materials etc.)   Publisher：The Society for Biotechnology, Japan   Joint Work  

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