論文 - 南茂 彩華
件数 11 件-
Nanmo, A; Suzuki, A; Kageyama, T; Fukuda, J
TISSUE ENGINEERING PART A 30 ( 15-16 ) S283 - S284 2024年8月 [査読有り]
担当区分：筆頭著者 記述言語：英語 掲載種別：研究論文（国際会議プロシーディングス） 共著
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Large-Scale Preparation of Hair Follicle Germs Using a Microfluidic Device
Sugiyama Ellen, Nanmo Ayaka, Nie Xiaolei, Chang Shu-Yung, Hashimoto Michinao, Suzuki Atsushi, Kagey … 全著者表示
Sugiyama Ellen, Nanmo Ayaka, Nie Xiaolei, Chang Shu-Yung, Hashimoto Michinao, Suzuki Atsushi, Kageyama Tatsuto, Fukuda Junji 閉じる
ACS Biomaterials Science & Engineering 10 ( 2 ) 998 - 1005 2024年1月 [査読有り]
DOI Web of Science PubMed CiNii Research
担当区分：筆頭著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：American Chemical Society 共著
Hair follicle morphogenesis during embryonic development is driven by the formation of hair follicle germs (HFGs) via interactions between epithelial and mesenchymal cells. Bioengineered HFGs are potential tissue grafts for hair regenerative medicine because they can replicate interactions and hair follicle morphogenesis after transplantation. However, a mass preparation approach for HFGs is necessary for clinical applications, given that thousands of de novo hair follicles are required to improve the appearance of a single patient with alopecia. In this study, we developed a microfluidics-based approach for the large-scale preparation of HFGs. A simple flow-focusing microfluidic device allowed collagen solutions containing epithelial and mesenchymal cells to flow and generate collagen microbeads with distinct Janus structures. During the 3 days of culture, the collagen beads contracted owing to cellular traction forces, resulting in collagen- and cell-dense HFGs. The transplantation of HFGs into nude mice resulted in highly efficient de novo hair follicle regeneration. This method provides a scalable and robust tissue graft preparation approach for hair regeneration.
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Bioprinting of hair follicle germs for hair regenerative medicine *
Nanmo Ayaka, Yan Lei, Asaba Tomoki, Wan Licheng, Kageyama Tatsuto, Fukuda Junji
Acta Biomaterialia 165 50 - 59 2023年6月 [査読有り]
DOI Web of Science PubMed CiNii Research
担当区分：筆頭著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Elsevier Science Ltd. 共著
Hair regenerative medicine is a promising approach to treat hair loss. The replication of in vivo tissue con-figurations and microenvironments, such as hair follicle germs, has been studied to prepare tissue grafts for hair regenerative medicine. However, such approaches should be scalable, because a single patient with alopecia requires thousands of tissue grafts. In this paper, we propose an approach for the scalable and automated preparation of highly hair-inductive tissue grafts using a bioprinter. Two collagen droplets (2 & mu;L each) containing mesenchymal and epithelial cells were placed adjacent to each other to fabricate hair-follicle-germ-like grafts. During three days of culture, the pairs of microgel beads were spontaneously contracted by cell traction forces, whereas the two cell types remained separated, where the densities of the cells and collagen were enriched more than 10 times. This approach allowed us to fabricate submil-limeter objects printed with millimeter-order accuracy, facilitating scalable and automated tissue graft preparation. Because of mesenchymal-epithelial interactions, hair microgels (HMGs, i.e., collagen-and cell-enriched microgels) efficiently regenerate hair follicles and shafts when transplanted into the back skin of mice. However, the generated hair shafts mostly remain under the skin. Therefore, we printed microgel beads onto surgical suture guides arrayed on a stage. The microgel beads were contracted along with the suture guides in culture prior to transplantation. The guide-inserted HMGs significantly im-proved hair-shaft sprouting through the skin, owing to the control of the orientation of the HMGs trans-planted into the skin. This approach is a promising strategy to advance hair regenerative medicine.Statement of significance This study proposes an approach for the scalable and automated preparation of highly hair-inductive grafts using a bioprinter. Two collagen droplets containing mesenchymal and epithelial cells were placed adjacently. Cell traction forces caused the pairs of microgel beads to spontaneously contract in culture. Because of mesenchymal-epithelial interactions, hair microgels (HMGs) efficiently regenerated hair fol-licles on the back skin of mice. However, the generated hair shafts remained mostly beneath the skin. Therefore, we printed microgel beads onto surgical suture guides arrayed on a stage. The guide-inserted HMGs significantly improved hair-shaft sprouting through the skin owing to the control of the orientation of the HMGs in the skin. This approach represents a promising strategy for advancing hair regenerative medicine.& COPY; 2022 The Author(s). Published by Elsevier Ltd on behalf of Acta Materialia Inc. This is an open access article under the CC BY license ( http://creativecommons.org/licenses/by/4.0/ )
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The role of lipids in promoting hair growth through HIF-1 signaling pathway
Seo, J; Matsumoto, K; Nanmo, A; Tu, S; Jeong, D; Chun, YS; Yan, L; Kageyama, T; Fukuda, J
SCIENTIFIC REPORTS 15 ( 1 ) 2025年2月 [査読有り]
記述言語：英語 掲載種別：研究論文（学術雑誌） 共著
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ヘアビーズにおけるヒト毛乳頭細胞の毛髪誘導能に対するPI3K/Aktシグナル伝達経路の効果
山根 萌奈実, Jieun Seo, Yinghui Zhou, 浅場 智貴, Shan Tu, 南茂 彩華, 景山 達斗, 福田 淳二
生物工学会誌 102 ( 2 ) 65 - 65 2024年2月
記述言語：日本語 掲載種別：研究論文（その他学術会議資料等） 出版者・発行元：公益社団法人 日本生物工学会 共著
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Exosomes for hair growth and regeneration
Zhou, YH; Seo, J; Tu, S; Nanmo, A; Kageyama, T; Fukuda, J
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 137 ( 1 ) 1 - 8 2024年1月 [査読有り]
記述言語：英語 掲載種別：研究論文（学術雑誌） 共著
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In vitro hair follicle growth model for drug testing
Kageyama Tatsuto, Miyata Hikaru, Seo Jieun, Nanmo Ayaka, Fukuda Junji
Scientific Reports 13 ( 1 ) 4847 2023年3月 [査読有り]
DOI Web of Science PubMed CiNii Research
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Nature Portfolio 共著
In vitro models of human hair follicle-like tissue could be fundamental tools to better understand hair follicle morphogenesis and hair drug screening. During prenatal development and postnatal cyclic hair regeneration, hair follicle morphogenesis is triggered by reciprocal interactions and the organization of the epithelial and mesenchymal cell populations. Given this mechanism, we developed an approach to induce hair peg-like sprouting in organoid cultures composed of epithelial and mesenchymal cells. Human fetal/adult epithelial and mesenchymal cells were cultured in a medium supplemented with a low concentration of either Matrigel or collagen I. These extracellular matrices significantly enhanced the self-organization capabilities of the epithelial and mesenchymal cells, resulting in spherical aggregation and subsequent hair peg-like sprouting. The length of the hair peg sprouting and associated gene expression significantly increased in the presence of a well-known hair drug, minoxidil. This approach may be beneficial for testing hair growth-promoting drug candidates.
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Hypoxia inducible factor-1a promotes trichogenic gene expression in human dermal papilla cells
Seo Jieun, Yan Lei, Kageyama Tatsuto, Nanmo Ayaka, Chun Yang-Sook, Fukuda Junji
Scientific Reports 13 ( 1 ) 1478 2023年1月 [査読有り]
DOI Web of Science PubMed CiNii Research
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Nature Portfolio 共著
Dermal papilla cells (DPCs) play critical roles in hair follicle development, but the underlying mechanisms that contribute to hair regeneration have yet to be fully elucidated, particularly in terms of alterations in androgenetic alopecia patients. In this study, we demonstrated that hypoxia-inducible factor-1 alpha (HIF-1 alpha) is suppressed in scalp tissues of androgenetic alopecia patients and potentially associated with hair follicle development. Using RT-qPCR and western blot, we found that mRNA and protein levels of trichogenic genes, LEF1 and versican (VCAN), were attenuated in HIF-1 alpha knockdown DPCs. Under an in vivo mimicked environment in a three-dimensional spheroid culture, HIF-1 alpha-suppressed DPCs downregulated the expression of hair induction-related genes. Finally, treatment with a HIF-1 alpha activator resulted in the elevated expression of trichogenic genes in DPCs. This study highlights the importance of dermal HIF-1 alpha expression in regulating trichogenic genes and provides a promising therapeutic target and a fundamental tissue engineering approach for hair loss treatment.
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Yamane Monami, Seo Jieun, Zhou Yinghui, Asaba Tomoki, Tu Shan, Nanmo Ayaka, Kageyama Tatsuto, Fukud … 全著者表示
Yamane Monami, Seo Jieun, Zhou Yinghui, Asaba Tomoki, Tu Shan, Nanmo Ayaka, Kageyama Tatsuto, Fukuda Junji 閉じる
Journal of Bioscience and Bioengineering 134 ( 1 ) 55 - 61 2022年6月 [査読有り]
DOI Web of Science PubMed CiNii Research
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Society for Biotechnology, Japan 共著
Dermal papilla cells (DPCs), which play a central role in the regulation of hair follicle development and hair growth, are among the most promising cell sources for hair regenerative medicine. However, a critical issue in the use of DPCs is the immediate loss of hair inducing functions in typical two-dimensional (2D) culture. We have previously demonstrated that when DPCs are encapsulated in drops of collagen gel (named hair beads, HBs), the density of collagen and cells is concentrated >10-fold during 3 d of culture through the spontaneous constriction of the drops, leading to efficient hair follicle regeneration upon transplantation. However, the mechanisms responsible for the activation of the hair-inducing functions of DPCs have been poorly elucidated. Here, transcriptome comparisons of human DPCs in HB culture and in typical 2D culture revealed that the phosphoinositide 3-kinase and Akt (PI3K/Akt) signaling pathway was significantly upregulated in HB culture. Inhibition of the PI3K/Akt signaling pathway decreased the hair-inducing capability of DPCs in HBs, while the activation of the PI3K/Akt signaling pathway using an activator improved trichogenous gene expression of DPCs in 2D culture. These results suggest that the PI3K/Akt signaling pathway is crucial for the maintenance and restoration of hair inductivity of DPCs. HB culture and/or activators of the PI3K/Akt signaling pathway could be a promising strategy for preparing DPCs for hair regenerative medicine. (c) 2022, The Society for Biotechnology, Japan. All rights reserved.
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Zhou Yinghui, Yamane Monami, Suzuki Kohei, Nanmo Ayaka, Tu Shan, Kageyama Tatsuto, Fukuda Junji
Alternatives to Animal Testing and Experimentation 27 ( 1 ) 1 - 13 2022年 [査読有り]
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：日本動物実験代替法学会 共著
<p>Hair follicle development and cycle are regulated by the reciprocal interactions between epithelial and mesenchymal cells. Mechanisms responsible for the regulation have mainly been studied using experimental animals such as rodent fetuses. The purpose of this study is to understand such communications by using <i>in vitro</i> cell culture models of human hair follicle-derived cells. Human dermal papilla cells (DPCs) were cultured using 2-dimensional (2D), spheroid (3D), and spheroid microwell array cultures in an oxygen-permeable substrate (3D-oxy). Exosomes secreted in the culture media were collected and purified. All three types of exosomes moderately promoted proliferation of human hair follicle stem cells (HFSCs). Exosomes from the 3D-oxy culture most efficiently upregulated the expression of CD200, CD34, and K15 in HFSCs compared to those from the other two culture methods. To investigate the effects of exosomes on <i>in vitro</i> hair follicle formation, mouse embryonic epithelial and mesenchymal cells were used to prepare hair follicle organoids. The expression analysis of trichogenic and HFSC marker genes revealed that exosomes from the 3D-oxy culture were the most effective in maintaining stem cells and stimulating hair follicle sprouting <i>in vitro</i>. Exosomes from human DPCs may have substantial potential for treating patients with hair loss.</p>
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Kageyama, T; Nanmo, A; Yan, L; Nittami, T; Fukuda, J
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 130 ( 6 ) 666 - 671 2020年12月 [査読有り]
記述言語：英語 掲載種別：研究論文（学術雑誌） 共著