研究者詳細 - Seo Jieun

Considerable global demand exists for the development of novel drugs for the treatment of alopecia. A recent report demonstrated that oxytocin promotes hair growth activity in human dermal papilla (DP) cells; however, its application in drugs or cosmetic products is challenging because rapid degradation and relatively large molecular weight prevent long-term topical administration on the scalp. Here, we examined cinnamic acid, a small molecule activator for oxytocin receptor (OXTR) expression. Treatment with cinnamic acid led to upregulation of OXTR and trichogenic gene expression in human DP cells. Furthermore, inhibition of OXTR with an antagonist, L-371,257, suppressed hair growth-related gene expression in DP cells. These findings suggest that cinnamic acid enhances the hair growth ability of DP cells via oxytocin signaling. Additionally, we tested the hair growth-promoting effects of cinnamic acid using hair follicle organoids in vitro and observed that cinnamic acid significantly promoted the growth of hair peg-like sprouting. These promising results may be useful for developing hair growth-promoting products targeting oxytocin.

ヘアビーズにおけるヒト毛乳頭細胞の毛髪誘導能に対するPI3K/Aktシグナル伝達経路の効果

山根萌奈実, Jieun Seo, Yinghui Zhou, 浅場智貴, Shan Tu, 南茂彩華, 景山達斗, 福田淳二

生物工学会誌 102 ( 2 ) 65 - 65 2024年2月

DOI CiNii Research

記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：公益社団法人日本生物工学会共著

Exosomes for hair growth and regeneration

Zhou, YH; Seo, J; Tu, S; Nanmo, A; Kageyama, T; Fukuda, J

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 137 ( 1 ) 1 - 8 2024年1月 [査読有り]

DOI Web of Science PubMed

記述言語：その他外国語掲載種別：研究論文（学術雑誌）共著

Exosomes for hair growth and regeneration

Zhou Yinghui, Seo Jieun, Tu Shan, Nanmo Ayaka, Kageyama Tatsuto, Fukuda Junji

Journal of Bioscience and Bioengineering 137 ( 1 ) 1 - 8 2023年12月

CiNii Research

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Society for Biotechnology, Japan 共著

Exosomes are lipid bilayer vesicles, 30-200 nm in diameter, that are produced by cells and play essential roles in cell-cell communication. Exosomes have been studied in several medical fields including dermatology. Hair loss, a major disorder that affects people and sometimes causes mental stress, urgently requires more effective treatment. Because the growth and cycling of hair follicles are governed by interactions between hair follicle stem cells (HFSCs) and dermal papilla cells (DPCs), a better understanding of the mechanisms responsible for hair growth and cycling through exosomes may provide new insights into novel treatments for hair loss. In this review, we focused on the comprehensive knowledge and recent studies on exosomes in the field of hair development and regeneration. We classified exosomes of several cellular origins for the treatment of hair loss. Exosomes and their components, such as microRNAs, are promising drugs for effective hair loss treatment.

Targeted down-regulation of Hipp1 ameliorates tau-induced deficits in Drosophila melanogaster

Sung Yeon Park, Jieun Seo, Seulbee Lee, Joohyung Kim, Sang Jeong Kim, Seungbok Lee, Yang-Sook Chun

Genes & Diseases 10 ( 6 ) 2248 - 2251 2023年11月 [査読有り]

DOI Web of Science PubMed

記述言語：英語掲載種別：研究論文（学術雑誌）単著

Effects of oxytocin on the hair growth ability of dermal papilla cells

Kageyama, T; Seo, J; Yan, L; Fukuda, J

SCIENTIFIC REPORTS 13 ( 1 ) 2023年10月 [査読有り]

DOI Web of Science PubMed

記述言語：英語掲載種別：研究論文（学術雑誌）共著

Effects of oxytocin on the hair growth ability of dermal papilla cells

Kageyama Tatsuto, Seo Jieun, Yan Lei, Fukuda Junji

Scientific Reports 13 ( 1 ) 2023年10月

CiNii Research

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Nature Portfolio 共著

Oxytocin (OXT) is a neuropeptide hormone termed "love hormone" produced and released during childbirth and lactation. It is also produced in response to skin stimulation (e.g., during hugging and massaging) and music therapy. The effects of OXT on various organs have been revealed in recent years; however, the relationship between hair follicles and OXT remains unclear. In this study, we examined the effects of OXT on dermal papilla (DP) cells that control hair growth by secreting growth/regression signals. Gene expression analysis revealed that DP signature markers were significantly upregulated in DP cells treated with OXT. In addition, we tested the hair growth-promoting effects of OXT using in vitro hair follicle organoids. OXT promoted the growth of hair peg-like sprouting by upregulating the expression of growth-promoting factors, including genes encoding vascular endothelial growth factor A (VEGFA). This study highlights the positive effects of OXT in hair follicles and may assist in the development of new treatments for alopecia.

Palmitoylation-driven PHF2 ubiquitination remodels lipid metabolism through the SREBP1c axis in hepatocellular carcinoma

Jeong, D; Park, JW; Kim, KS; Kim, J; Huh, J; Seo, J; Kim, YL; Cho, JY; Lee, KW; Fukuda, J; Chun, YS

NATURE COMMUNICATIONS 14 ( 1 ) 2023年10月 [査読有り]

DOI Web of Science PubMed

記述言語：その他外国語掲載種別：研究論文（学術雑誌）共著

Palmitoylation-driven PHF2 ubiquitination remodels lipid metabolism through the SREBP1c axis in hepatocellular carcinoma

Jeong Do-Won, Park Jong-Wan, Kim Kyeong Seog, Kim Jiyoung, Huh June, Seo Jieun, Kim Ye Lee, Cho Joo … 全著者表示

Nature Communications 14 ( 1 ) 2023年10月

CiNii Research

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Nature Portfolio 共著

Palmitic acid (PA) is the most common fatty acid in humans and mediates palmitoylation through its conversion into palmitoyl coenzyme A. Although palmitoylation affects many proteins, its pathophysiological functions are only partially understood. Here we demonstrate that PA acts as a molecular checkpoint of lipid reprogramming in HepG2 and Hep3B cells. The zinc finger DHHC-type palmitoyltransferase 23 (ZDHHC23) mediates the palmitoylation of plant homeodomain finger protein 2 (PHF2), subsequently enhancing ubiquitin-dependent degradation of PHF2. This study also reveals that PHF2 functions as a tumor suppressor by acting as an E3 ubiquitin ligase of sterol regulatory element-binding protein 1c (SREBP1c), a master transcription factor of lipogenesis. PHF2 directly destabilizes SREBP1c and reduces SREBP1c-dependent lipogenesis. Notably, SREBP1c increases free fatty acids in hepatocellular carcinoma (HCC) cells, and the consequent PA induction triggers the PHF2/SREBP1c axis. Since PA seems central to activating this axis, we suggest that levels of dietary PA should be carefully monitored in patients with HCC.

Ulmus davidiana 60% edible ethanolic extract for prevention of pericyte apoptosis in diabetic retinopathy

Kim Iljin, Seo Jieun, Lee Dong Hyun, Kim Yo-Han, Kim Jun-Hyung, Wie Myung-Bok, Byun Jun-Kyu, Yun Ja … 全著者表示

FRONTIERS IN ENDOCRINOLOGY 14 2023年5月 [査読有り]

DOI Web of Science PubMed

記述言語：英語掲載種別：研究論文（学術雑誌）共著

In vitro hair follicle growth model for drug testing

Tatsuto Kageyama, Hikaru Miyata, Jieun Seo, Ayaka Nanmo & Junji Fukuda

Scientific Reports 13 ( 1 ) 4847 2023年3月 [査読有り]

DOI Web of Science PubMed CiNii Research

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Nature Portfolio 単著

In vitro models of human hair follicle-like tissue could be fundamental tools to better understand hair follicle morphogenesis and hair drug screening. During prenatal development and postnatal cyclic hair regeneration, hair follicle morphogenesis is triggered by reciprocal interactions and the organization of the epithelial and mesenchymal cell populations. Given this mechanism, we developed an approach to induce hair peg-like sprouting in organoid cultures composed of epithelial and mesenchymal cells. Human fetal/adult epithelial and mesenchymal cells were cultured in a medium supplemented with a low concentration of either Matrigel or collagen I. These extracellular matrices significantly enhanced the self-organization capabilities of the epithelial and mesenchymal cells, resulting in spherical aggregation and subsequent hair peg-like sprouting. The length of the hair peg sprouting and associated gene expression significantly increased in the presence of a well-known hair drug, minoxidil. This approach may be beneficial for testing hair growth-promoting drug candidates.

Hypoxia inducible factor-1α promotes trichogenic gene expression in human dermal papilla cells

Jieun Seo, Lei Yan, Tatsuto Kageyama, Ayaka Nanmo, Yang-Sook Chun & Junji Fukuda

Scientific Reports 13 ( 1 ) 1478 2023年1月 [査読有り]

DOI Web of Science PubMed CiNii Research

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Nature Portfolio 単著

Dermal papilla cells (DPCs) play critical roles in hair follicle development, but the underlying mechanisms that contribute to hair regeneration have yet to be fully elucidated, particularly in terms of alterations in androgenetic alopecia patients. In this study, we demonstrated that hypoxia-inducible factor-1 alpha (HIF-1 alpha) is suppressed in scalp tissues of androgenetic alopecia patients and potentially associated with hair follicle development. Using RT-qPCR and western blot, we found that mRNA and protein levels of trichogenic genes, LEF1 and versican (VCAN), were attenuated in HIF-1 alpha knockdown DPCs. Under an in vivo mimicked environment in a three-dimensional spheroid culture, HIF-1 alpha-suppressed DPCs downregulated the expression of hair induction-related genes. Finally, treatment with a HIF-1 alpha activator resulted in the elevated expression of trichogenic genes in DPCs. This study highlights the importance of dermal HIF-1 alpha expression in regulating trichogenic genes and provides a promising therapeutic target and a fundamental tissue engineering approach for hair loss treatment.

Effects of the PI3K/Akt signaling pathway on the hair inductivity of human dermal papilla cells in hair beads

Monami Yamane, Jieun Seo, Yinghui Zhou, Tomoki Asaba, Shan Tu, Ayaka Nanmo, Tatsuto Kageyama, Junj … 全著者表示

Journal of Bioscience and Bioengineering 134 ( 1 ) 55 - 61 2022年7月 [査読有り]

DOI Web of Science PubMed CiNii Research

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Elsevier {BV} 共著

Dermal papilla cells (DPCs), which play a central role in the regulation of hair follicle development and hair growth, are among the most promising cell sources for hair regenerative medicine. However, a critical issue in the use of DPCs is the immediate loss of hair inducing functions in typical two-dimensional (2D) culture. We have previously demonstrated that when DPCs are encapsulated in drops of collagen gel (named hair beads, HBs), the density of collagen and cells is concentrated >10-fold during 3 d of culture through the spontaneous constriction of the drops, leading to efficient hair follicle regeneration upon transplantation. However, the mechanisms responsible for the activation of the hair-inducing functions of DPCs have been poorly elucidated. Here, transcriptome comparisons of human DPCs in HB culture and in typical 2D culture revealed that the phosphoinositide 3-kinase and Akt (PI3K/Akt) signaling pathway was significantly upregulated in HB culture. Inhibition of the PI3K/Akt signaling pathway decreased the hair-inducing capability of DPCs in HBs, while the activation of the PI3K/Akt signaling pathway using an activator improved trichogenous gene expression of DPCs in 2D culture. These results suggest that the PI3K/Akt signaling pathway is crucial for the maintenance and restoration of hair inductivity of DPCs. HB culture and/or activators of the PI3K/Akt signaling pathway could be a promising strategy for preparing DPCs for hair regenerative medicine. (c) 2022, The Society for Biotechnology, Japan. All rights reserved.

Lipid metabolic reprogramming by hypoxia-inducible factor-1 in the hypoxic tumour microenvironment

Seo Jieun, Yun Jeong-Eun, Kim Sung Joon, Chun Yang-Sook

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY 474 ( 6 ) 591 - 601 2022年6月 [査読有り]

DOI Web of Science PubMed

記述言語：英語掲載種別：研究論文（学術雑誌）共著

Tumor-intrinsic FABP5 is a novel driver for colon cancer cell growth via the HIF-1 signaling pathway

Seo Jieun, Yun JeongEun, Fukuda Junji, Chun Yang-Sook

CANCER GENETICS 258-259 151 - 156 2021年11月 [査読有り]

DOI Web of Science PubMed CiNii Research

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Elsevier Science Inc. 共著

Dysfunctional lipid metabolism is a known cause of cancer development and progression, yet little is known about the underlying molecular mechanisms that contribute to cancer progression. In this study, we demonstrate that fatty acid binding protein 5 (FABP5) is elevated in colon cancer tissue and this increased expression is linked to upregulation of the hypoxia-inducible factor-1 (HIF-1) signaling pathway. Under physiologically in vivo mimicked conditions via a polydimethylsiloxane (PDMS)-based three-dimensional (3D) culture chip, FABP5-knockdown colon cancer cells exhibited attenuated cell growth throughout the culture period. FABP5 was found to regulate HIF-lot protein levels and gene expression levels within the HIF-1 alpha signaling pathway under hypoxic conditions. Our results provide evidence that supports the use of FABP5 as a prognostic factor in colon cancer. The FABP5/HIF-1 alpha axis is a promising target for ameliorating fatty acid-triggered cancer progression. (C) 2021 The Author(s). Published by Elsevier Inc.

Metastasis-on-a-chip reveals adipocyte-derived lipids trigger cancer cell migration via HIF-1 alpha activation in cancer cells

Seo Jieun, Kim Kyeong Seog, Park Jong-Wan, Cho Joo-Youn, Chang Hak, Fukuda Junji, Hong Ki Yong, Chu … 全著者表示

BIOMATERIALS 269 120622 2021年2月

DOI Web of Science PubMed CiNii Research

記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：Elsevier Science Ltd. 共著

Although obesity is a newly considered risk factor for cancer, the mechanisms by which adipocyte-derived metabolites accelerate cancer malignancy have yet to be elucidated. To identify the connection among heterogeneous cell types, conventional methods including Transwell assays or conditioned media (CM) have been used; however, these methods do not fully reflect niche effects in the tumor microenvironment (TME). Here, we established an oxygen permeable polydimethylsiloxane (PDMS)-based three-dimensional (3D) culture system to allow direct attachment between human adipocyte derived stem cells (ADSCs) and cancer cells. By doing so, a physiologically bioactive TME was created, which could be used to reveal further the relationships between different cell types. We found that co-culture of cancer cells with ADSCs resulted in a dispersion phenomenon, and the dispersed spheroid was well matched with the enhanced metastatic potential of cancer cells. Lipid profiling and in vitro migration assays suggested that lipids are the driving force for cancer cell migration via HIF-1 alpha upregulation. In addition, the lipid/HIF-1 alpha axis promoted tumor metastasis in a xenograft mouse model. This study presents an in vitro model of a biomimetic TME and provides new mechanistic insights into the effects of ADSC-released fatty acids on cancer cells as oncometabolites.

Fatty-acid-induced FABP5/HIF-1 reprograms lipid metabolism and enhances the proliferation of liver cancer cells

Seo Jieun, Jeong Do-Won, Park Jong-Wan, Lee Kwang-Woong, Fukuda Junji, Chun Yang-Sook

COMMUNICATIONS BIOLOGY 3 ( 1 ) 1 2020年10月

DOI Web of Science PubMed CiNii Research

記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：Nature Research 共著

Hypoxia-inducible factor-1 alpha (HIF-1 alpha) is a transcription factor essential for cancer cell survival. The reprogramming of lipid metabolism has emerged as a hallmark of cancer, yet the relevance of HIF-1 alpha to this process remains elusive. In this study, we profile HIF-1 alpha -interacting proteins using proteomics analysis and identify fatty acid-binding protein 5 (FABP5) as a critical HIF-1 alpha -binding partner. In hepatocellular carcinoma (HCC) tissues, both FABP5 and HIF-1 alpha are upregulated, and their expression levels are associated with poor prognosis. FABP5 enhances HIF-1 alpha activity by promoting HIF-1 alpha synthesis while disrupting FIH/HIF-1 alpha interaction at the same time. Oleic-acid treatment activates the FABP5/HIF-1 alpha axis, thereby promoting lipid accumulation and cell proliferation in HCC cells. Our results indicate that fatty-acid-induced FABP5 upregulation drives HCC progression through HIF-1-driven lipid metabolism reprogramming. Seo et al. identify fatty acid-binding protein 5 (FABP5) as a booster of HIF-1 alpha activity. They find that oleic-acid treatment activates the FABP5/HIF-1 alpha axis, promoting lipid accumulation and cell proliferation in liver cancer cells. This study provides insights into how fatty acids drive the progression of cancer.

Neddylation blockade induces HIF-1 alpha driven cancer cell migration via upregulation of ZEB1

Park Jun Bum, Seo Jieun, Park Jong-Wan, Chun Yang-Sook

SCIENTIFIC REPORTS 10 ( 1 ) 2020年10月

DOI Web of Science PubMed

記述言語：日本語掲載種別：研究論文（学術雑誌）共著

Molecular mechanism of neddylation-dependent suppression of cancer cell migration

Chun Yang-Sook, Seo Jieun, Park Jun Bum

CANCER RESEARCH 80 ( 16 ) 2020年8月

DOI Web of Science

記述言語：日本語掲載種別：研究論文（学術雑誌）共著

Neddylation of sterol regulatory element-binding protein 1c is a potential therapeutic target for nonalcoholic fatty liver treatment

Ju Uk-Il, Jeong Do-Won, Seo Jieun, Park Jun Bum, Park Jong-Wan, Suh Kyung-Suk, Kim Jae Bum, Chun Ya … 全著者表示

CELL DEATH & DISEASE 11 ( 4 ) 2020年4月

DOI Web of Science PubMed

記述言語：日本語掲載種別：研究論文（学術雑誌）共著

Histone demethylase PHF2 activates CREB and promotes memory consolidation

Hye‐Jin Kim, Sung Won Hur, Jun Bum Park, Jieun Seo, Jae Jin Shin, Seon‐Young Kim, Myoung‐Hwan Kim, … 全著者表示

EMBO reports 20 ( 9 ) 2019年9月 [査読有り]

DOI

記述言語：英語掲載種別：研究論文（学術雑誌）単著

Targeted downregulation of kdm4a ameliorates tau-engendered defects in Drosophila melanogaster

Sung Yeon Park, Jieun Seo, Yang-Sook Chun

Journal of Korean Medical Science 34 ( 33 ) 2019年8月 [査読有り]

DOI

記述言語：英語掲載種別：研究論文（学術雑誌）単著

The E3 ligase C-CBL inhibits cancer cell migration by neddylating the proto-oncogene c-Src

Gun-Woo Lee, Jun Bum Park, Sung Yeon Park, Jieun Seo, Seung-Hyun Shin, Jong-Wan Park, Sang Jung Kim … 全著者表示

Oncogene 37 ( 41 ) 5552 - 5568 2018年10月 [査読有り]

DOI

記述言語：英語掲載種別：研究論文（学術雑誌）単著

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