Faculty of Engineering, Division of Materials Science and Chemical Engineering

Job Title


Research Fields, Keywords

mouse, reproduction, sperm, germ cell, RNA biology, monoclonal antibody

Mail Address

E-mail address

Related SDGs

The Best Research Achievement in Research Career 【 display / non-display

The Best Research Achievement in the last 5 years 【 display / non-display

Graduating School 【 display / non-display


    Toho University   Faculty of Science   Department of Biology   Graduated

Graduate School 【 display / non-display


    Kobe University  Graduate School, Division of National Science and Technology (except Gakushuin University, Konan University)  Doctor Course  Completed


    Toho University  Graduate School, Division of Natural Science  Master Course  Completed

Degree 【 display / non-display

  • Doctor of Science -  Kobe University

Campus Career 【 display / non-display

  • 2011.04

    Duty   Yokohama National UniversityFaculty of Engineering   Division of Materials Science and Chemical Engineering   Professor  

  • 2009.04

    Duty   Yokohama National UniversityResearch Institute of Environment and Information Sciences   Professor  

  • 2007.04

    Duty   Yokohama National UniversityResearch Institute of Environment and Information Sciences   Associate Professor  

  • 2006.02

    Duty   Yokohama National UniversityResearch Institute of Environment and Information Sciences   Associate Professor  

  • 2001.04

    Duty   Yokohama National UniversityResearch Institute of Environment and Information Sciences   Research Associate  

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External Career 【 display / non-display

  • 1999.05

    University of Pennsylvania, Center for Research on Reproduction and Women's Health, Visiting Faculty   Research Associate  

  • 1989.04

    National Institute of Radiological Sciences,   Department of Biology   Researcher  

  • 1988.04

    Japan Society for the Promotion of Science,   Research Fellow  

Field of expertise (Grants-in-aid for Scientific Research classification) 【 display / non-display

  • Molecular biology


Books 【 display / non-display

  • Genetics in Wild Mice, Its Application to Biomedical Research

    H. Suzuki and Y. Kurihara (Part: Joint Work )

    Japan Scientific Societies Press  1994

  • Wild Mice in Immunology, Current Topics in Microbiology and Immunology

    K. Moriwaki, N. Miyashita, H. Suzuki, Y. Kurihara and H. Yonekawa (Part: Single Work , Range: Genetic Features of Major Geographical Isolates of Mus musculus )

    Springer Verlag  1986

Papers 【 display / non-display

  • Rapid and reliable hybridoma screening method that is suitable for production of functional structure-recognizing monoclonal antibody.

    Atsumi Sakaguchi, Chika Nakajima, Ayuko Sawano, Yoichiro Tanaka, and Yasuyuki Kurihara

    J.Biosci. Bioeng. ( Elsevier )    2021.02  [Refereed]

    Joint Work

     View Summary

    Monoclonal antibodies are extremely valuable functional biomaterials that are widely used not only in life science research but also in antibody drugs and test drugs. There is also a strong need to develop high-quality neutralizing antibodies as soon as possible in order to stop the rapid spread of new infectious diseases such as the SARS-CoV-2 virus. This study has developed a membrane-type immunoglobulin-directed hybridoma screening (MIHS) method for obtaining high-quality monoclonal antibodies with high efficiency and high speed. In addition to these advantages, this paper demonstrates that the MIHS method can selectively obtain monoclonal antibodies that specifically recognize the functional structure of proteins. The MIHS method is a useful technology that greatly contributes to the research community because it can be easily introduced in any laboratory that uses a flow cytometer.


  • Coxfa4l3, a novel mitochondrial electron transport chain Complex 4 subunit protein, switches from Coxfa4 during spermatogenesis.

    Masahiro Endou, Kaito Yoshida, Makoto Hirota, Chika Nakajima, Atsumi Sakaguchi, Naoto Komatsubara,Y … Show more authors

    Mitochondrion ( Elsevier )  52   1 - 7   2020.02  [Refereed]

    Joint Work

     View Summary

    We identified Coxfa4l3, previously called C15orf48 or Nmes1, as a novel accessory protein of Complex IV of the mitochondrial electron transport chain (ETC). Amino acid sequence comparison, the intracellular localization and the protein expression data showed that the protein is the third isoform of Coxfa4 and the expression of Coxfa4 and Coxfa4l3 proteins during spermatogenesis showed a mutually exclusive pattern, implying that Coxfa4 replaces Coxfa4l3 in Complex IV after meiosis. These results may provide some insight into the unique mechanism of ATP production in late spermatogenesis.

    DOI PubMed

  • Flow cytometry-based method for rapid and high-throughput screening of hybridoma cells secreting monoclonal antibody


    Journal of Bioscience and Bioengeering   125 ( 4 )   464 - 469   2018.05  [Refereed]

    Joint Work

    DOI PubMed

  • Dynamic paraspeckle component localization during spermatogenesis

    Andrew T Major , Cathryn A Hogarth , Julia C Young, Yasuyuki Kurihara, David A Jans and Kate L Love … Show more authors

    Society for reproduction and fertility ( Bioscientifica Ltd )  Vol.153 ( 3 )   267 - 280   2019.09  [Refereed]

    Joint Work


  • Specific intron-dependent loading of DAZAP1 onto the cox6c transcript suppresses pre-mRNA splicing efficacy and induces cell growth retardation

    Kenta Sasaki, Mana Ono, Kaori Takabe, Atsushi Suzuki, Yasuyuki Kurihara

    GENE     2018  [Refereed]

    Joint Work


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Review Papers 【 display / non-display

  • Mechanism of mammalian germ cell development: toward new assisted reproductive technologies

    Atsushi Suzuki, Yumiko SAGA

    Strides of medicine   238 ( 13 ) 1221 - 1223   2011.09

    Introduction and explanation (scientific journal)   Joint Work


Presentations 【 display / non-display

  • Mitochondrial Biogenesis and the Activity Regulation by Posttranscriptional Control of cox6c mRNA by an RNA Binding Protein, Prrp

    Yasuyuki Kurihara, Hiroyuki Sakagami, Mana Ono, Kaori Takabe, Kenta Sasaki, Yuki Tasaka, Chiharu Yanai, Atsuhiro Yasuhara

    International Symposium on Mitochondria 2014  (Tokyo)  2014.11.06  

  • Specific interaction of the nuclear transporter importin alpha 2 with paraspeckle protein 1 modulates nuclear paraspeckle formation

    A Major, Hogarth CA, Miyamoto Y, Sarraj MA, Smith CL, Koopman P, Kurihara Y, Jans DA, Loveland KA

    The 12th International Symposium on Spermatology   (Australia)  2011.08.10  

Preferred joint research theme 【 display / non-display

  • Reproduction mammalian genetics