KURIHARA Yasuyuki

Affiliation

Faculty of Engineering, Division of Materials Science and Chemical Engineering

Job Title

Professor

Research Fields, Keywords

RNA biology, mouse, monoclonal antibody, germ cell, reproduction, sperm

Mail Address

E-mail address

Related SDGs




The Best Research Achievement in Research Career 【 display / non-display

The Best Research Achievement in the last 5 years 【 display / non-display

Education 【 display / non-display

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    1989.3

    Kobe University   Doctor Course   Completed

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    1986.3

    Toho University   Master Course   Completed

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    1984.3

    Toho University   Department of Biology   Graduated

Degree 【 display / non-display

  • Doctor of Science - Kobe University

Campus Career 【 display / non-display

  • 2011.4
     
     

    Duty   Yokohama National UniversityFaculty of Engineering   Division of Materials Science and Chemical Engineering   Professor  

  • 2009.4
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    2011.3

    Duty   Yokohama National UniversityResearch Institute of Environment and Information Sciences   Professor  

  • 2007.4
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    2009.3

    Duty   Yokohama National UniversityResearch Institute of Environment and Information Sciences   Associate Professor  

  • 2006.2
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    2007.3

    Duty   Yokohama National UniversityResearch Institute of Environment and Information Sciences   Associate Professor  

  • 2001.4
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    2006.1

    Duty   Yokohama National UniversityResearch Institute of Environment and Information Sciences   Research Assistant  

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External Career 【 display / non-display

  • 1999.5
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    2000.3

    University of Pennsylvania, Center for Research on Reproduction and Women's Health, Visiting Faculty   Research Associate  

  • 1989.4
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    1992.7

    National Institute of Radiological Sciences,   Department of Biology   Researcher  

  • 1988.4
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    1989.3

    Japan Society for the Promotion of Science,   Research Fellow  

Academic Society Affiliations 【 display / non-display

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    日本分子生物学会

Research Areas 【 display / non-display

  • Life Science / Molecular biology

 

Research Career 【 display / non-display

  • ミトコンドリア電子伝達系の活性制御

    Project Year:

  • 迅速簡便なモノクローナル抗体作製法の開発

    Project Year:

  • 哺乳動物の生殖細胞の発生分化に関わるRNA情報の発現制御の研究

    Project Year:

Books 【 display / non-display

  • 小笠原100の素顔Ⅱ

    東京農大小笠原100の素顔編集委員会( Role: Other)

    東京農大出版会  ( ISBN:4-88694-042-0

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    Language:Japanese Book type:General book, introductory book for general audience

  • 制限酵素、「ヒドロラーゼ」

    栗原靖之、上杉晴一( Role: Joint author)

    廣川書店 

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    Language:Japanese Book type:Scholarly book

  • 高感度蛍光色素を使ったポストステイニングによる蛍光ディファレンシャルディスプレイ(SSDD)法、「non‐RI実験の最新プロトコール 蛍光の原理と実際:遺伝子解析からバイオイメージングまで」

    丸本佳代子、栗原靖之、( Role: Joint author)

    羊土社 

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    Language:Japanese Book type:Scholarly book

  • タンパク質と核酸のゲル電気泳動の高感度蛍光染色剤を使った染色法、「non‐RI実験の最新プロトコール 蛍光の原理と実際:遺伝子解析からバイオイメージングまで」

    明神玲子、栗原靖之、( Role: Joint author)

    羊土社 

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    Language:Japanese Book type:Scholarly book

  • Genetics in Wild Mice, Its Application to Biomedical Research

    H. Suzuki and Y. Kurihara ( Role: Joint author)

    Japan Scientific Societies Press 

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    Language:Japanese Book type:Scholarly book

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Papers 【 display / non-display

  • Rapid and reliable hybridoma screening method that is suitable for production of functional structure-recognizing monoclonal antibody.

    Atsumi Sakaguchi, Chika Nakajima, Ayuko Sawano, Yoichiro Tanaka, and Yasuyuki Kurihara

    J.Biosci. Bioeng.   2021.2  [Reviewed]

    DOI

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier   Joint Work  

    Monoclonal antibodies are extremely valuable functional biomaterials that are widely used not only in life science research but also in antibody drugs and test drugs. There is also a strong need to develop high-quality neutralizing antibodies as soon as possible in order to stop the rapid spread of new infectious diseases such as the SARS-CoV-2 virus. This study has developed a membrane-type immunoglobulin-directed hybridoma screening (MIHS) method for obtaining high-quality monoclonal antibodies with high efficiency and high speed. In addition to these advantages, this paper demonstrates that the MIHS method can selectively obtain monoclonal antibodies that specifically recognize the functional structure of proteins. The MIHS method is a useful technology that greatly contributes to the research community because it can be easily introduced in any laboratory that uses a flow cytometer.

  • Coxfa4l3, a novel mitochondrial electron transport chain Complex 4 subunit protein, switches from Coxfa4 during spermatogenesis.

    Masahiro Endou, Kaito Yoshida, Makoto Hirota, Chika Nakajima, Atsumi Sakaguchi, Naoto Komatsubara,Y … Show more authors

    Mitochondrion   52   1 - 7   2020.2  [Reviewed]

    DOI PubMed

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier   Joint Work  

    We identified Coxfa4l3, previously called C15orf48 or Nmes1, as a novel accessory protein of Complex IV of the mitochondrial electron transport chain (ETC). Amino acid sequence comparison, the intracellular localization and the protein expression data showed that the protein is the third isoform of Coxfa4 and the expression of Coxfa4 and Coxfa4l3 proteins during spermatogenesis showed a mutually exclusive pattern, implying that Coxfa4 replaces Coxfa4l3 in Complex IV after meiosis. These results may provide some insight into the unique mechanism of ATP production in late spermatogenesis.

    Other Link: https://reader.elsevier.com/reader/sd/pii/S1567724919301060?token=A6C41278D44D96F052B1804055705E0896D7F97ADFF1FEAFA255600765FCFEB9C7279514356C5F6F81D7FAE8F46BEFDC

  • Flow cytometry-based method for rapid and high-throughput screening of hybridoma cells secreting monoclonal antibody

    赤城幸、中嶋千佳、田中陽一郎、栗原靖之

    Journal of Bioscience and Bioengeering   125 ( 4 )   464 - 469   2018.5  [Reviewed]

    DOI PubMed

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    Language:English   Publishing type:Research paper (scientific journal)   Joint Work  

  • Dynamic paraspeckle component localization during spermatogenesis

    Andrew T Major , Cathryn A Hogarth , Julia C Young, Yasuyuki Kurihara, David A Jans and Kate L Love … Show more authors

    Society for reproduction and fertility   Vol.153 ( 3 )   267 - 280   2019.9  [Reviewed]

    DOI

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Bioscientifica Ltd   Joint Work  

    Other Link: https://rep.bioscientifica.com/view/journals/rep/158/3/REP-19-0139.xml

  • Specific intron-dependent loading of DAZAP1 onto the cox6c transcript suppresses pre-mRNA splicing efficacy and induces cell growth retardation

    Kenta Sasaki, Mana Ono, Kaori Takabe, Atsushi Suzuki, Yasuyuki Kurihara

    GENE   2018  [Reviewed]

    DOI

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    Language:English   Publishing type:Research paper (scientific journal)   Joint Work  

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Review Papers 【 display / non-display

  • 陰陽のミトコンドリア 第1回ミトコンドリアは面白い

    First Knowledge 第7号   ( 7 )   3 - 6   2018.8

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (bulletin of university, research institution)   Publisher:株式会社 jast  

  • Mechanism of mammalian germ cell development: toward new assisted reproductive technologies

    Atsushi Suzuki, Yumiko SAGA

    Strides of medicine   238 ( 13 )   1221 - 1223   2011.9

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   Joint Work  

  • 実験がうまくいく 蛍光・発光試薬の選び方と使い方、 「タンパク質検出法」

    羊土社   2007.10

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (other)   Joint Work  

  • ハツカネズミ亜種間雑種がもたらす染色体不安定性

    アニメイト   2006

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   Single Work  

Grant-in-Aid for Scientific Research 【 display / non-display

  • 分子病理学的アプローチに基づく男性不妊症に関わるミトコンドリア電子伝達系制御機構

    2021.4 - 2023.3

    科学研究費補助金  Grant-in-Aid for Scientific Research(C)

    Investigator(s):栗原靖之

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    Grant type:Competitive

    申請者を含めた多くの研究により、男性不妊症の約8割を占める造精障害ではミトコンドリア電子伝達系(Mt-ETC) の機能不全が起こっていることが示されている。この申請課題は、精子形成後期に特異的に発現するETC複合体Ⅳタンパク質(Coxfa4l3)の欠損(KO)マウスを、世界で初めての男性不妊症疾患モデル動物として利用して、男性不妊症が起こる分子メカニズムを分子病理学的アプローチで迫り、この治療法の開発に貢献する。
    さらに、男性不妊症の有望な検査薬候補としてETC複合体Ⅳタンパク質を使った簡便な検査法の確立を目指す。これにより、多くの不妊に悩む人達に貢献したい。

Presentations 【 display / non-display

  • ミトコンドリアは面白い!ミトコンドリア未病をご存じですか?

    栗原 靖之  [Invited]

    予防医学研究会公開セミナー  ㈱JAST 教育部門

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    Event date: 2018.5

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:厚生会館5階  

    今日の健康を明日へつなぐ。
    ミトコンドリアと仲良く!

  • 蛍光抗原によるB細胞標識とそのモノクローナル抗体作製法の改良への応用

    赤城幸、栗原靖之  [Invited]

    第38回日本分子生物学会年会ランチョンセミナー 

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    Event date: 2015.12

    Language:The in addition, foreign language   Presentation type:Oral presentation (invited, special)  

    我々が開発したモノクローナル抗体作製法について紹介した。

  • 高等生物での遺伝情報発現の主役RNA

    栗原靖之  [Invited]

    平成27年度 第1回セミナー  神生研

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    Event date: 2015.7

    Language:The in addition, foreign language   Presentation type:Oral presentation (invited, special)  

    Venue:横浜  

    RNA制御が高等生物の高次生命現象に深く関わっていることをわかりやすく講義した。

  • Mitochondrial Biogenesis and the Activity Regulation by Posttranscriptional Control of cox6c mRNA by an RNA Binding Protein, Prrp

    Yasuyuki Kurihara, Hiroyuki Sakagami, Mana Ono, Kaori Takabe, Kenta Sasaki, Yuki Tasaka, Chiharu Yanai, Atsuhiro Yasuhara

    International Symposium on Mitochondria 2014 

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    Event date: 2014.11

    Language:English   Presentation type:Poster presentation  

    Venue:Tokyo  

  • RNA結合タンパク質Prrpはpre-mRNA splicingの上位因子か

    栗原靖之  [Invited]

    Waterfront Seminor  産総研

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    Event date: 2012.10

    Language:The in addition, foreign language   Presentation type:Oral presentation (invited, special)  

    Venue:東京  

    Prrpが一つのmRNAの複数個のイントロンスプライシングに関わる可能性について論じた。

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Preferred joint research theme 【 display / non-display

  • 新規モノクローナル抗体の作製

  • Reproduction mammalian genetics

 

Charge of on-campus class subject 【 display / non-display

  • 2022   Molecular Biology

    College of Engineering Science

  • 2022   Cell Genetics

    College of Engineering Science

  • 2022   Biology of Phenome

    Graduate school of Engineering Science

  • 2022   Modern Biology 1

    College of Engineering Science

  • 2022   Seminar D in Chemistry Applications and Life Science

    Graduate school of Engineering Science

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Charge of off-campus class subject 【 display / non-display

  • 医科学実験法Ⅰ、Ⅱ

    Institution:横浜市立大学

 

Committee Memberships 【 display / non-display

  • 国立大学法人 機器分析センター協議会

    2020.4 - 2024.3  会長

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    Committee type:Other